高岭石固定化微生物对菲的降解研究

Biodegradation of phenanthrene by Kaolinite immobilized a degradation bacteria

从新疆克拉玛依油田受污染土壤中分选筛出1株能够以菲为唯一碳源和能源生长的细菌(命名为XJB4),初步鉴定为苍白杆菌(Ochrobactrum sp.)。在初始pH 7.0,培养温度30℃,投菌比例10%时,菌株XJB4对菲(初始浓度50 mg/L)的降解率在9 d内达88%以上。采用吸附法可以将菌株XJB4固定在高岭石上,固定化微生物具有传质性好、耐毒害能力强特点,对菲的降解在72 h低浓度条件下可达到95.24%,优于游离菌对菲的去除率70.12%。

A bacteria strain XJB4 which ergy was isolated from Kelamayi oil field mount of bacteria for phenanthrene biod can utilize phenanthrene as the sole source carbon source and en- in Xinjiang autonomous region. The optimum pH, temperature, a- egradation by XJIM was found to be 7.0,30 μ ,10% respective- ly,and the degradation rate of phenanthrene was up to 88% within 9 d. The method of strain XJB4 ab- sorbed on Kaolinite showed that immobilized microorganism has the characteristic of good transfer and poi- son resistance, the degradation of phenanthrene within 72 h under low concentration can achieve 95.24% ,however,the removal rate of free bacteria was 70.12%.