黄豆、豆浆、豆渣中黄豆苷、染料木苷、染料木素的含量测定

Determination of daidzein,genistin and genistein in soybean,soybean milk and bean dreg

建立同时测定黄豆中黄豆苷、染料木苷、染料木素的RP-HPLC法,为豆类食品中以上有效化学成分的检测和合理食用提供参考。样品经80%甲醇超声提取15 min,采用Eclipse XDB-C_(18)(4.6 mm×250 mm,5.0μm)色谱柱,以甲醇∶0.1%磷酸=62.5∶37.5为流动相,流速为1.00 mL/min,进样量为10μL,检测波长为254 nm。以峰面积计算结果为黄豆苷、染料木苷、染料木素分别在0.5~32.0 mg/L,4.0~128.0 mg/L,0.1~16.0 mg/L呈良好线性关系,相关系数r均为0.999 9;测定黄豆苷、染料木苷、染料木素的高、中、低浓度精密度均<2.0%,三成分的重现性精密度分别为1.67%,0.06%,0.07%,表明该方法精密度和重现性良好。测定黄豆样品中黄豆苷,染料木苷,染料木素的平均含量分别为724.3,1 306.7,16.0μg/g。黄豆苷在其豆浆和豆渣中含量分别为黄豆的39.6%,23.2%;染料木苷在其豆浆、豆渣中分别为黄豆的53.5%,34.9%;染料木素在豆渣中含量约占黄豆的43.1%,而豆浆中因浓度低而未检出。

To establish a RP-HPLC method for the simultaneous determination of daidzein, genistin, genistein in soybean, soybean milk and bean dreg, which provide a reference for beans above the active chemical components of detection and reasonable use. After the samples were extracted with 80% metha- nol by ultrasonic treatment for 15 rain ,they were analysed by RP-HPLC with Eclipse XDB-C1s （4.6 mm × 250 mm,5.0 μm） column under methanol： 0.1% phosphoric acid =62.5：37.5 as the mobile phase at a flow rate of 1.00 mL/min. The injection volume was 10 μL and the detection was UV detector at 254 nm. The daidzein in 0.5 - 32.0 mg/L,genistin in 4.0 - 128.0 mg/L,genistein in 0.1 - 16.0 mg/L showed a good linear correlation coefficient being 0. 999 9 by peak area calculation. The accuracies of daidzein, genistin, genistein in high, medium and low concentrations are all less than 2.0% and repeatability preci- sion of three-component were 1.67% ,0.06%, and 0.07%, respectively. The results indicated that the method show good precision and reproducibility. The average content of daidzein, genistin and genistein in soybeans were 724.3,1 306.7 and 16.0 p,g/g, respeetively. The daidzein content in its soy milk, bean dreg were 39.6%, 23.2% and the genistin content were 53.5%, 34.9% of soybeans, respectively. Genistein in bean dreg was 43.1% of soybeans, but no signal was detected in its soy milk due to the low concentration.