基于CLP败血症小鼠中补体C5a与白细胞介素-6相关性研究

Study on the correlation of complement C5a with IL-6 in CLP septicemic mice

目的:探讨败血症发病机制中炎症介质C5a与细胞因子白细胞介素-6(IL-6)的关系。方法:利用经典盲肠结扎穿孔术(CLP)建立败血症动物模型,在败血症活动期,取不同时间段建模组和假手术组小鼠的腹腔灌洗液,检测补体C5a和IL-6的表达。结果:建模组小鼠的腹腔灌洗液中补体C5a和IL-6水平在建模后第4、6、12、24 h均明显高于假手术组(P<0.01);伴随病变恢复,2组实验动物体内补体C5a和IL-6的表达水平逐渐趋于正常。结论:CLP败血症小鼠发病机制中,炎症介质C5a与细胞因子IL-6的调节有一定的线性相关趋势。

Objective：To explore the relationship between inflammatory mediator C5a and intedeukin-6 （IL-6） in the pathogenesis of sepsis. Methods：The animal model of sepsis was established using the cecal ligation and puncture（CLP）. During the active stage of sepsis,the expressions of completement C5a and IL-6 in peritoneal lavage fluid of the different time-points model group and sham operation group were detected. Results：The levels of the complement C5a and IL-6 in peritoneal lavage fluid in model group after 4,6, 12 and 24 h of establishing model were significantly higher than those in sham group （ P 〈 0.01 ）. With the recovery of disease, the levels of complement C5a and IL-6 in two groups gradually returned to the normal level. Conclusions：During the pathogenesis of CLP sepsis in mice ,the inflammatory mediators C5a and IL-6 have a linear correlation trend.