Aurora kinases在宫颈癌发病机制中的探索

Study on the Pathogenesis of Aurora Kinases in Cervical Cancer

目的通过分析Aurora激酶A、B在正常宫颈组织,CIN及宫颈癌组织中的表达,探讨Aurora激酶A、B在宫颈癌发生、发展中的作用。方法 (1)选取深圳市30例宫颈癌患者,我院CIN患者30例,宫颈健康者30例,并取组织标本作研究。(2)采用荧光定量法测定Aurora激酶A、B在不同宫颈组织的表达及与HPV16、、HPV18感染关系。(3)采用细胞仪分析30例宫颈癌患者组织标本中Aurora激酶A、B在表达与不表达时细胞的增殖率,凋亡率,并对患者采用化疗药物顺铂治疗后观察癌细胞的增殖率,凋亡率。结果 HPV16、HPV18及Aurora激酶在正常宫颈组织中的表达情况较低,HPV16、HPV18在宫颈癌中表达情况极高,表达率分别为96.67%、100.00%,CIN中表达其次,表达率分别为66.67%、70.00%,约占致癌型别的70%左右,差异具有统计学意义(P<0.05)。Aurora激酶A在宫颈癌组织标本存在高表达,Aurora激酶B高表达与肿瘤的恶性侵袭相关,差异具有统计学意义(P<0.05)。观察组宫颈癌细胞中Aurora激酶A、Aurora激酶B表达情况低于对照组,差异均具有统计学意义(P<0.05)。对宫颈癌患者采用Aurora激酶抑制剂VX-680或化疗药物顺铂治疗后,癌细胞的增殖率显著下降、凋亡率显著升高,Aurora激酶未表达组癌细胞增殖率低于表达组、凋亡率高于表达组,差异具有统计学意义(P<0.05)。结论Aurora激酶在女性宫颈癌细胞中存在显著高表达的现象,通过对宫颈癌患者采用Aurora激酶抑制剂VX-680或化疗药物顺铂治疗后,宫颈癌细胞的增殖率显著下降、凋亡率显著升高。

Objective To investigate the expression of Aurora kinase A and B in normal cervical tissues,CIN and cervical cancer tissues,and to explore the role of Aurora kinase A and B in the genesis and development of cervical carcinoma.Methods A total of 30 patients with cervical cancer in Shenzhen were selected,and a total of CIN patients in our hospital in were selected,and a total of 30 patients with cervical cancer were enrolled in this study.Fluorescence quantitative method was used to detect the expression of Aurora kinase A and B in different cervical tissues and the relationship with HPV16,HPV18 infection.The FCM analysis of 30 cases of patients with cervical cancer tissue specimens of Aurora kinase A,B expression and the expression of cell proliferation rate,apoptosis rate,and for patients with cisplatin was observed after treatment of cancer cell proliferation rate,apoptosis rate.Results The expression of HPV16,HPV18 and Aurora kinase in normal cervical tissues.The low expression of high HPV16 and HPV18 in cervical cancer,the expression rates were 96.67%,100.00%,followed by the expression of CIN,expression rates were 66.67%,70.00%,accounting for about 70% of the left and right type of cancer,the difference was statistically significant（P〈0.05）.Aurora kinase A was highly expressed in cervical cancer tissues,and the high expression of Aurora kinase B was associated with malignant invasion of tumor.The difference was statistically significant（P〈0.05）.The expression of Aurora kinase A and Aurora kinase B in cervical cancer cells in the observation group was significantly lower than that in the control group,the difference was statistically significant（P〈0.05）.For patients with cervical cancer by Aurora kinase inhibitor VX-680 or cisplatin treatment,cancer cell proliferation rate decreased significantly,the apoptosis rate was significantly increased,Aurora kinase expression group not cancer cell proliferation rate is lower than the expression group,apoptosis rate was higher than that of group,the differences were statistically significant（P〈0.05）.Conclusion Aurora kinase significantly high expression of the phenomenon in women cervical cancer cells,the cervical cancer patients with Aurora kinase inhibitor VX-680 or cisplatin treatment,cervical cancer cell proliferation rate decreased significantly,the apoptosis rate was significantly increased.