激光扫描共聚焦显微镜在植物研究中的应用

Application of Laser Scanning Confocal Microscopy in Plant Research

荧光成像技术、光学显微镜和计算机成像分析系统的完美结合催生了激光扫描共聚焦显微镜(LSCM)的出现,其优越的结构特点实现了将较厚样品能够逐点逐层扫描成清晰图像,有利于各种荧光信号的采集,直观进行三维重建和样品多重荧光信号采集进行的定量分析。这些优势功能极大地提高了从细胞、分子水平来探求生物时空表达基本功能的能力。本文详细阐述了近年来随着该项技术发展的日益多样化,激光扫描共聚焦显微镜技术在植物组织化学、植物细胞器和细胞骨架、植物发育方面的应用。

Fluorescence imaging technology, optical microscope and computer image analysis system spawned a perfect combination of laser scanning confocal microscope （LSCM） , and its superior structure features to achieve the thicker sample could be scanned from point to layer into a clear image, which was conducive to all kinds of fluorescence signal acquisition, three-dimensional reconstruction and quantitative analysis of sample the multiplex fluorescence signal acquisition. These advantages greatly enhanced the ability to explore the basic functions of biological space-time expression from the cellular and molecular levels. Laser scanning confocal microscopy were reviewed in the fields of plant tissue chemistry, plant organelles and cytoskeleton, plant development and so on, with the increasing diversification of the technology,.