盐碱地塔宾曲霉菌的解磷能力及其对小麦生长的影响

Phosphate-solubilizing Ability of Aspergillus Tubingensis and Its Effects on Growth of Wheat in Seedling Stage

[目的]研发新型解磷生物菌肥,提高黄河三角洲盐碱障碍耕地作物产量。[方法]采用无机磷液体培养基培养的方法,从黄河三角洲盐碱化菜园根际土壤筛选得到一株解磷真菌CT1,即塔滨曲霉菌(Aspergillus tubingensis),对其解磷能力进行了深入研究。[结果]解磷菌CT1的解磷能力随发酵液盐浓度升高降低,当发酵液盐浓度在0.03%~6%时,发酵液中有效磷浓度可维持在523.5~338.5mg/L,且解磷菌的溶磷量与发酵液pH之间存在明显的负相关。解磷菌CT1在葡萄糖作为碳源的培养基上生长状况最好,在(NH_4)_2SO_4作为氮源的培养基上生长状况最好。接入解磷菌15d的小麦与未接菌的小麦相比,茎长增加了16.24%,茎鲜重增加了12.35%,根长增加了21.6%。[结论]解磷菌CT1对盐碱地小麦幼苗生长有一定的促进作用,可作为提高盐碱地作物产量的新型解磷生物菌肥利用。

[Objective]The objective of the study is to research a new biological phosphorus fertilizer in order to improve crop yield in alkaline soil in the Yellow River Delta.[Methods]The cultivating method used in this study was inorganic phosphorus liquid culture medium.Fungi Aspergillus tubingensis（CT1）was screened from the rhizosphere soil in the garden of Yellow River delta.We researched the phosphate-solubilizing ability of fungi.[Results]The phosphate-solubilizing ability of fungi CT1 was reduced with the increasing of fermented liquid salt concentration.When the concentration of NaCl was between 0.03% and6%,the concentration of available phosphorus was maintained between 523.5and 338.5mg/L.There was an obvious correlation between the available phosphorus concentration and fermented liquid pH vale.CT1 could grow normally under four different kinds of carbon sources,and the glucose was the best culture medium.CT1 could grow normally under four different kinds of nitrogen source,and（NH_4）_2SO_4 was the best medium.Compared with uninoculated wheat,fungi CT1 inoculated wheat,after 15 days of wheat cultivation,the length of the stem increased by 16.24%,the fresh weight of the stem increased by 12.35%,and the root length increased by 21.6%.[Conclusion]Phosphate solubilizing bacteria CT1 could promote cropyield of saline-alkali soil,and can be used as a new phosphate-solubilizing fertilizer.