木薯25个WRKY家族转录因子在生物胁迫下的表达分析

Expression Analysis of 25 WRKY Transcription Factors in Cassava under Biotic Stresses

根据拟南芥和水稻的WRKY基因和木薯基因组序列,利用生物信息学方法预测木薯Me WRKY转录因子家族成员,并对其进行系统进化关系和保守结构域的分析。通过病原菌接种处理,分析不同Me WRKY转录因子的表达差异。结果显示,木薯共编码25个与抗病相关WRKY蛋白,在病原菌胁迫条件下,其中16个Me WRKY基因的表达受到显著影响,表明这些WRKY蛋白可能参与木薯的防御应答反应。

WRKY family is one of the largest families of transcription factors in higher plants. The WRKY transcription factors play a central role in signal networks and plant growth. The WRKY transcription factors have been extensively studied in response to biotic stresses in model plants, but little information about their roles in cassava is available. According to the WRKY sequences of Arabidopsis and rice, and cassava genome sequences, 25 WRKY genes in cassava were predicted and analyzed including their phylogenetic relationships and conserved domains. Expression profiles of these genes in response to pathogen were analyzed by quantitative PCR. The results showed that the 25 identified MeWRKY genes in cassava had the conserved WRKY domain and zinc-finger structure and had distinct expression patterns in response to pathogen infection, out of which 16 were significantly up-or down-regulated, implying that these members might participate in defense response to biotic stresses in cassava.