利用DNAStar软件预测热带无爪螨主要致敏原Blo t 5的抗原表位

Prediction of Eiptopes of the Major Allergen Blo t 5 from Blomia tropicalis by Using DNAStar Software

利用DNAStar Protean软件对热带无爪螨主要致敏原Blo t 5的二级结构和表面特性进行分析,如理化性质、亲水性、表面可及性、可塑性等,预测Blo t 5蛋白的B抗原表位和T抗原表位。实验结果表明,Blo t 5蛋白的二级结构以α螺旋为主,存在6个潜在的B抗原表位位点,可能的B抗原表位区域有:18-31,39-48,56-77,86-101,105-112,117-133氨基酸残基或其附近。含有7个潜在的T细胞抗原表位区域:32-37,40-44,57-65,70-79,86-95,100-104,115-120氨基酸残基或其附近。笔者应用Protean软件预测了热带无爪螨主要致敏原Blo t 5的抗原表位,为进一步研究Blo t 5致敏原的结构与功能,研制针对热带螨的预防性和治疗性疫苗、开发相应的诊断试剂盒奠定了基础。

The secondary structure and surface properties of Blot 5 allergen from Blomia tropicalis, such as physical and chemical characters, hydrophilicity, surface probability, flexible regions and antigenicity, were analyzed by using the DNAStar Protean software to predict B-cell and T-cell epitopes of Blot. 5. The secondary structure of Blo t 5 was rich in alpha helix, and the Blot 5 contained six potential B cell epitopes located at 18 - 31, 39 - 48, 56 - 77, 86 - 101,105 - 112, 117 - 133 amino acid residues or nearby. There were seven potential T cell epitopes located at 32 - 37, 40 - 44, 57 - 65, 70 - 79, 86 - 95, 100 - 104, 115 - 120 amino acid residues or nearby. The epitopes of the major allergen Blo t 5 from B. tropicalis were predicted by using the Protean software. The results can provide a base for the studies of structure and function of the allergen Blot 5, and development of preventive and therapeutic vaccines for Blomia tropicalis and diagnostic kits for detection of allergens induced by B. tropicalis.