掺锶磷酸钙骨水泥材料的生物学性能体外研究

Biological properties of strontium-doped calcium phosphate cement

目的拟探讨一种新型骨替代材料的生物学性能。方法选用本课题组研发的一种新型掺锶磷酸钙骨水泥,用细胞培养和电镜检测新型材料的生物学性能。结果不同浓度的材料浸提液培养的细胞形态均与正常对照组细胞无明显差别,L929小鼠成纤维胞在材料上能够黏附生长,培养24h后可见明显的细胞表面绒毛和细胞伸展,并可见细胞向材料表面的孔隙中生长。结论新研发的骨替代材料无细胞毒性,并具有良好的生物相容性。

Objective To investigate the biological properties of a new bone substitute material. Methods A new type of strontium-doped calcium phosphate cement was developed by the authors. The evaluation was performed by cell culture and electron microscope examination. Results The cells cultured by material leaching solution of different concentration were not evidently different from normal cells and had no cytotoxicity. L929 fibroblast could grow on the material. Cell villus and cell spreading were observed after 24 hours. The cells grew into the pore on material surface. Conclusion The results demonstrated that the new bone substitute material in this study has no eytotoxicity and can be considered as a biocompatible material.