足体调节蛋白SH3PXD2B基因生物信息学分析

Bioinformatics analysis of SH3PXD2B gene

目的利用多种生物信息学方法,研究SH3PXD2B基因序列信息和蛋白结构,为今后进一步验证该基因在口腔颌面头颈部的功能提供依据。方法从Genbank得到SH3PXD2B序列,采用基因结构分析,预测该蛋白的亚细胞定位、二级结构、三级结构、功能结构域和抗原表位等信息。结果 SH3PXD2B基因编码长度为911aa的蛋白,具有1个PX和4个SH3结构域,蛋白理论分子量为101579.0,理论等电点为8.82。蛋白结构中α螺旋占21.62%,β转角占6.70%,无规则卷曲占58.95%。蛋白结构中无信号肽,但有4个跨膜结构,和8处抗原表面位点。蛋白互作网络显示,与其互作蛋白多与细胞伪足形成和细胞粘附有关。进化分析显示家猪、家兔等与人SH3PXD2B基因相似性更高。结论利用生物信息学方法预测SH3PXD2B蛋白的结构和功能,能为其在口腔颌面头颈部功能的研究提供信息基础。

Objective To investigate the function and regulatory mechanism of human SH3PXD2B gene, aiming at providing evidence for further validation of the gene function in the oral and maxillofacial region. Methods Based on SH3PXD2B sequences from GenBank, the differences in various species, subcelluar location, secondary structure, functional domains and epitopes were analyzed by bioinformatics. Results The gene encoded a length of 911 amina acid protein with five functional domains, including 1 PX and 4 SH3 domain. The molecular weight was 101579.0, theoretical isoelectric point was 8.82, α-helix secondary structure accounted for 21.62% , β-fold for 6.70% , and random coil for 58.95%. There was no signal peptide in SH3PXD2B. However, 4 transmembrane and 8 epitopes structure were predicted in the sequence. Based on network, most interact proteins were related to pseudopodia formation and cell adhesion. Phylogenetic analysis revealed that SH3PXD2B genes of pig and rabbit were similar with human. Conclusion Structure and function prediction of human SH3PXD2B provided the basic information for further research in oral and maxillofacial-head and neck region.