水杨酸和不同糖浓度对马铃薯试管微薯形成与生长的影响研究

Effect of sucrose concentrations with or without SA on formation and growth of In vitro microtuber in potato

分别在水杨酸和非水杨酸诱导下 ,研究了克新 1号和青薯 1 68脱毒苗在不同蔗糖浓度诱导培养基中试管微薯的发育状况 ,结果表明 :水杨酸影响微薯形成率 ,而蔗糖影响膨大率 ,二者协同决定微薯数量和重量。无水杨酸诱导时 ,8%～ 1 0 %糖浓度诱导效果最好 ,单瓶微薯数达 2 0枚左右 ,单薯平均重量 2 0 0 mg;0 .5mmol/L水杨酸诱导时 ,1 0 %～ 1 2 %蔗糖浓度效果最佳 ,单瓶微薯数达 30～ 40枚 ,单薯平均重量超过 1 0 0 mg,有效微薯的绝对数量最多。

The formation and development of in vitro microtuber treated by SA and different sucrose concentrations are explored.The result show that,under the induction of non SA,8%~10% sucrose concentration is the best;0.5 mmol/L SA is in favour of increasing the number of microtuber while sucrose concentration is closely relevent to the size of microtuber.The best results are obtained when 10%~12% sucrose concentrations and 0.5 mmol/L SA are in the medium.The number of microtuber per vessel is 30~40 and the average weight per microtuber increased to more than 100 mg.