用基因枪法将抗除草剂基因导入小麦栽培品种的研究

Studies on the Transfer of Herbicide-resistance Gene into Cultivated Wheat by Biolistics

利用基因枪法将抗除草剂bar基因导入西南地区的 3个小麦栽培品种 ,共获得 7个转基因植株 ,转化频率在 0 .45 %～ 1 .2 %之间 ,转化周期缩短至 3个月左右。对抗性植株进行PCR和PCR_Southern杂交检测 ,初步确定bar基因已导入小麦基因组。做转基因植株叶片对除草剂PPT的抗性试验 ,有 4株呈抗性 ,3株呈部分抗性 ,表明bar基因已在小麦植株中得到表达。

A herbicide resistance gene bar was transferred into immature embryos of three wheat cultivars by biolistics. As a result,7 transformed plants were obtained from a total 902 bombardment embryos with the transformation frequency of 0.45～1.2% and transformation time was reduced to 3 months or so. PCR and PCR_Southern blot analysis suggested that the foreign gene bar had been transferred into wheat plants. The herbicide_resistance test indicated that four plants were totally resistant to PPT and the other three were partially resistant. It suggested that bar gene was expressed in wheat plants.