期刊文献+

白藜芦醇对血管紧张素Ⅱ诱导的成纤维细胞增殖的作用及其机制 被引量:2

Effect of resveratrol on the proliferation of NIH/3T3 induced by AngⅡ and related mechanism
下载PDF
导出
摘要 目的研究白藜芦醇(Resv)对血管紧张素Ⅱ(AngⅡ)诱导成纤维细胞增殖的作用和机制。方法体外培养小鼠胚胎成纤维细胞(NIH/3T3),建立AngⅡ诱导的成纤维细胞增殖模型。采用细胞增殖-毒性检测试剂盒(CCK-8)检测细胞活力,Western blot技术检测Ⅰ型胶原蛋白(COLⅠ)、Ⅲ型胶原蛋白(COLⅢ)、基质金属蛋白酶-1(MMP-1)和基质金属蛋白酶抑制因子-1(TIMP-1)的表达。结果与Control组相比,AngⅡ组NIH/3T3增殖明显,COLⅠ、COLⅢ、MMP-1和TIMP-1蛋白表达均显著增加,且TIMP-1/MMP-1增大;与AngⅡ组相比,加入10、20、30、100μmol/L白藜芦醇(Resv)对AngⅡ引起的NIH/3T3增殖有抑制作用(后期实验选用Resv的浓度为20μmol/L),COLⅠ、COLⅢ、MMP-1和TIMP-1蛋白表达明显减少,且TIMP-1/MMP-1降低;与AngⅡ组相比,AngⅡ预处理组引起的变化趋势同Resv组,强度不如Resv,且两者具有协同作用。结论白藜芦醇和AngⅡ预处理均可抑制AngⅡ引起的成纤维细胞增殖和活化,前者效果优于后者,两者合用效果更佳,其保护作用的机制与减少胶原蛋白的分泌和下调MMP-1与TIMP-1的比值有关。 Objective To explore the effect of resveratrol (Resv) on the proliferation of NIH/ 3T3 induced by Ang Ⅱ and related mechanism. Methods The fibroblast proliferation model was established by culturing NIH/3T3 with Ang Ⅱ. Cell viability was detected by cell counting kit-8 (CCK-8) reagent kit. Western blot assay was used to detect the expression of collagen I , collagen m, MMP-1 and TIMP-1. Results Compared with the normal group, the cell prolif- eration was increased and the expression of collagen Ⅰ-Ⅲ and MMP-1, TIMP-1 were significant- ly increased in Ang Ⅱ group ; Compared with the Ang Ⅱ group, the expression of collagen I , collagen m, MMP-1 and TIMP-1 and TIMP-1/MMP-1 was down regulated in Resv groups (the concentration of Resv was 10, 20, 30, 100 μmol/L and 20 p.moL/L was used in the following study). The trend of change induced by angiotensin Ⅱ preconditioning (APC) was similar with Resv group. Conclusion Resv and Ang Ⅱ could inhibit the proliferation and activation of myocardial fibroblasts induced by Ang Ⅱ , which mechanism is related to decreasing secretion of collagen and down regulating TIMP-1/ MMP-1.
出处 《哈尔滨医科大学学报》 CAS 2017年第1期8-12,共5页 Journal of Harbin Medical University
基金 国家自然科学基金资助项目(81270311)
关键词 精胺 乳鼠心肌细胞 氧化应激 活性氧 凋亡 缺氧-缺血 resveratrol proliferation angiotensin Ⅱ fibroblast MMP-1 TIMP-1
  • 相关文献

参考文献3

二级参考文献42

  • 1杜昕,易宗春,戴闺柱.血管紧张素Ⅱ和醛固酮对心脏成纤维细胞Ⅲ型胶原mRNA表达的影响[J].中华老年心脑血管病杂志,2000,2(1):45-47. 被引量:6
  • 2陈勇兵,陈如坤,陈力,吴明,施立,杨文涛,钱永跃.乳鼠心肌成纤维细胞培养方法的改进[J].江苏医药,2005,31(3):189-190. 被引量:8
  • 3Weber KT.Cardiac imerstitium in heart and disease:the fibrillar collagen network[J].J Am Coll Cardiol,1989,13(7):1637-1652.
  • 4Diep QN,Benkirane K,Amiri F,et al.PPAR alpha activatorfenoilbrate inhibits myocardial inflammation and fibrosis in angiotenain Ⅱ-infused rats[J].Mol Cell Cardiol,2004,36(2):295-304.
  • 5Samuel CS,Unemori EN,Mookerjee I,et al.Relaxin modulates eardiac fibroblast proliferation differenfiation and collagen production and reverses cardiac fibrosis in vivo[J].Endocrinology,2004,145(9):4125-4133.
  • 6Rossi G,Boscaro M.Ronconi V,et al.Aldosterone as a cardiovscular risk factor[J].Trends Endocrinol Metab,2005,16(3):104-107.
  • 7Racha R,Stier CT.Pathophysiological effects of aldostemne in cardiovasculartissues[J].Trends Endoerinol Metab,2001,12(7):308-314.
  • 8Beggah AT,Escoubet B,Puttini S,et al.Reversible cardiac fibrosis and heart failure induced by conditional expression of an antisense mRNA of the mineralocorticoid receptor in cardiomyocytes[J].PNAS,2002,99(10):7160-7165.
  • 9Delcayre C.Silvestre JS,Gamier A,et al.Cardiac aldostemne production and ventrieular remodeling[J].Kidney Int,2000,57(4):1346-1351.
  • 10satoh M,Nakamura M,Saitoh H,et al.Aldosterone synthase expression and myocardial fibrosis in the failing human heart[J].Clin Sci.2002,102(4):381-386.

共引文献37

同被引文献19

引证文献2

二级引证文献13

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部