常温机械灌注下大鼠骨髓间充质干细胞修复心脏死亡后捐献供肝

Bone marrow mesenchymal stem cells in repairing donor liver after cardiac death for liver under normothermic machine perfusion in rats

目的研究常温机械灌注（NMP）下大鼠骨髓间充质干细胞对心脏死亡后捐献（DCD）肝脏的修复作用。方法体外培养Wistar大鼠骨髓间充质干细胞（BMMSCs），建立心脏死亡后热缺血45min模型。30只Wistar大鼠随机分成NMP组、NMP＋BMMSCs（N＋B）组、冷保存（CS）组，每组（n=10）于2和4h检测相关指标。结果N＋B组在修复肝脏功能和肝脏病理方面，包括超微结构、改善灌注液酸性环境能力以及升高三磷酸腺苷水平等，优于NMP组和cs组（均P〈0．05）。NMP组和N＋B组耗氧量在2h后出现明显变化[2h：（24．35±0．64）ml／min比（29．33±0．47）ml／min；3h：（25．33±0．86）ml／min比（30．34±0．49）ml／min；4h：（26．88±1．07）ml／min比（31．76±0．96）ml／min；P〈0．05]，提示N＋B组的修复作用明显优于其他两组。结论在常温机械灌注下大鼠骨髓间充质干细胞可对DCD大鼠供肝发挥一定的修复作用。

Objective To study the repairing effect of bone marrow mesenchymal stem cells （BMMSCs） on the donor liver after cardiac death （DCD） under normal temperature mechanical pcrfusion （NMP） in rats. Methods BMMSCs of Wistar rats were cultured in vitro, and 45-min warm ischemia after cardiac death model was established. The 30 Wistar rats were randomly divided into NMP, NMP ＋ BMMSCs （ N ＋ B） , cold storage （CS） groups, and the parameters were detected at 2 h and 4 h （ n = 10）. Results N ＋ B group was superior to NMP group and CS group in repairing the liver function and liver pathology including ultrastructure, improving the perfusate acidic environment, and increasing adenosine triphosphate level （ P 〈 0.05 ）. The oxygen consumption of NMP group and N ＋ B group were significantly different after 2h [2 h： （24.35±0.64） ml/min vs. （29.33±0.47） ml/min; 3 h： （25.33 ±0.86） ml/min vs. （30. 34 ±0.49） ml/min; 4 h： （26.88 ±1.07） ml/min vs. （31.76 ±0.96） ml/min; P 〈0.05], suggesting that the liver condition in N ＋ B group was significantly better than that in the other two groups. Conclusion Bone marrow mesenchymal stem cells could obviously repair the DCD grafts under normal temperature mechanical perfusion.