miR-126-3p在甲状腺癌中的表达及其功能研究

Expression of miR-126-3p in thyroid cancer and function research

目的:探讨微小分子核糖核酸(miR)-126-3p在甲状腺癌中的表达,探讨其生物学功能。方法:RT-PCR检测35例甲状腺癌、癌旁组织以及三种甲状腺癌细胞系(TPC-1、FTC-133、8505C)中miR-126-3p表达量;将甲状腺癌细胞分为类似物组(mimic)和对照组(NC),分别转染miR-126-3p mimic及阴性对照质粒。两组细胞增殖、凋亡分别采用Brdu-ELISA法和流式细胞术检测,Transwell小室法检测两组细胞迁移和侵袭。结果:35例癌组织中miR-126-3p相对表达量显著低于癌旁组织(0.384±0.028 vs 0.981±0.039,t=10.291,P<0.05);在三种甲状腺癌细胞中,TPC-1细胞中miR-126-3p相对表达量最低;与NC组比较,mimic组甲状腺癌细胞TPC-1增殖受到显著的抑制,第3天两组间开始表现出统计学差异(P<0.05);与NC组比较,mimic组甲状腺癌细胞TPC-1凋亡显著增加[(15.32±3.20)%vs(8.12±1.17)%,t=4.623,P<0.05],迁移受到抑制(26.68±4.48 vs 82.21±3.65,t=17.789,P<0.05),侵袭受到抑制(12.28±1.03 vs 34.43±2.10,t=8.103,P<0.05)。结论:甲状腺癌组织中miR-126-3p表达降低,上调miR-126-3p表达可以显著抑制甲状腺癌细胞增殖、促进凋亡、抑制迁移和侵袭,miR-126-3p可能作为一种抑癌基因在甲状腺癌中发挥重要的生物学功能。

Objective：To investigate the expression of miR-126-3p in thyroid cancer and the biological function. Methods：The expression of miR-126-3p in thyroid carcinoma, adjacent tissues and three types of thyroid cancer cells （TPC-1, FTC-133,8505C） were detected by RT-PCR;thyroid cancer ceils were divided into analogue group（ mimic ） and control group （NC）, which were respectively with the transfection of miR-126-3p mimic and negative control plasmid. The proliferation and apoptosis in two groups were respectively detected by Brdu-ELISA and flow cytometry. The migration and invasion were detected by Transwell Chambers method. Results：The ex- pression of miR-126-3p in thyroid carcinoma was significantly lower than adjacent tissues（0. 384＋0. 028 vs 0. 981 -＋0. 039, t = 10. 291, P〈0. 05 ） ;the expression of miR-126-3p in TPC-1 was the lowest among three types of thyroid cancer cells. Compared with NC group, the proliferation of TPC-1 in mimic group was significantly inhibited, the same with migration（ 26.68±4. 48 vs 82. 21 ±3.65, t = 17. 789, P〈0. 05 ） and invasion （ 12. 28± 1. 03 vs 34.43 ±2. I0, t = 8. 103, P〈0.05 ）, which the apoptosis was significantly increased [ （ 15.32±3.20） % vs （8.12±1.17）% ,t =4. 623,P〈0. 051- Conclusion：The miR-126-3p expression is reduced in thyroid cancer tissue, over- expression of miR-126-3p significantly suppresses the proliferation, migration and invasion of thyroid cancer cells, and promotes the ap- optosis ,miR-126-3p can play an important biological function as a cancer suppressor gene in thyroid cancer.