晚期糖基化终末产物诱导皮肤成纤维细胞中lncRNA表达谱的变化

Expression profiles of long noncoding RNA in advanced glycosylation end products treated dermal fibroblast

目的探讨长链非编码RNA(lncRNA)在晚期糖基化终末产物(AGEs)诱导的皮肤成纤维细胞与正常皮肤成纤维细胞中表达谱的差异。方法利用lncRNA芯片技术检测AGEs诱导的皮肤成纤维细胞与正常皮肤成纤维细胞中lncRNA表达谱的变化,经过对原始数据进行标准化处理后,筛选出差异表达lncRNA。结果与正常培养的皮肤成纤维细胞相比较,24 h AGEs诱导的皮肤成纤维细胞中差异表达的lncRNA有3421条,其中1079条表达上调,2342条表达下调,差异表达达10倍以上的lncRNA有448条,其中208条表达上调,240条表达下调。结论与正常皮肤成纤维细胞相比较,AGEs诱导的皮肤成纤维细胞的lncRNA的表达谱发生了显著的变化,提示lncRNA可能参与了糖尿病皮肤病变的发生、发展。

Objective To explore the expression profile variation of long noncoding RNA （IneRNA） in advanced glycosylation end products （AGEs） treated dermal fibroblast compared with normal dermal fibroblast. Methods The differences of lncRNA expression profile between AGEs treated fibroblast and normal fibroblast were analyzed through IncRNA microarray. LneRNA with differential expression were screened out after pretreatment of raw data. Results Compared with normal fibroblast, 3421 lncRNAs were identified to be different expression （fold change≥2.0） in AGEs treated fibroblast. 1079 were up-regulated and 2342 were down-regulated. 448 lncRNAs （208 up-regulated and 240 down-regulated ） were highly differentially expressed with absolute fold change greater than ten. Conclusion The expression profile of IneRNA in AGEs treated fibroblast was significantly changed in comparsion with normal fihroblast. These lncRNA may be involved in the occurrence and development of diabetic dermopathy.