复发性流产外周IL-10^+Tim-3^+ T细胞降调节

Down regulatory effect of peripheral IL-10^+Tim-3^+T cells in recurrent spontaneous abortion patients

目的探讨外周血CD3+T细胞中T细胞免疫球蛋白黏蛋白-3(Tim-3)及程序性死亡因子-1(PD-1)联合细胞因子在复发性流产(RSA)中的诊断价值。方法用流式细胞术检测19例RSA患者及17例正常早孕者外周血CD3^+T细胞表面Tim-3及PD-1含量,以及破细胞膜后细胞内因子干扰素(IFN)-γ和白介素(IL)-10在Tim-3^+PD-1^+、Tim-3^-PD-1^-、Tim-3^-PD-1^+和Tim-3^+PD-1^-4群细胞内的表达情况。结果 RSA患者Tim-3^+PD-1^+T细胞比例(0.57%±0.26%)明显低于早孕组(1.24%±0.77%)(P<0.001)。RSA组4群T细胞中IL-10阳性细胞所占比例分别为33.55%±16.27%、0.92%±0.88%、1.61%±1.35%、16.36%±13.98%;早孕组4群T细胞中IL-10阳性细胞占比分别为45.92%±17.89%、0.49%±0.27%、0.92%±0.68%、33.43%±16.98%。RSA组和正常组Tim-3^+PD-1^+T细胞群中IL-10的含量均显著高于其他3群(P<0.05);RSA组Tim-3^+PD-1^+和Tim-3^+PD-1^-T细胞中IL-10阳性细胞含量(33.55%±16.27%,16.36%±13.98%)显著低于正常早孕组(45.92%±17.89%,33.43%±16.98%)(P<0.05,P<0.01)。而IFN-γ在RSA组和正常组4群细胞中的表达无统计学差异。结论 RSA患者IL-10^+Tim-3^+T细胞显著降低,可作为判断RSA的新的参考指标。

ObjectiveTo evaluate the diagnosis value of T cell immunoglobin and mucin-3 （Tim-3） and programmed death-1 （PD-1） combined with cytokines on peripheral CD3^＋T cells in the patients of recurrent spontaneous abortion （RSA）.MethodsFlow cytometry was used to detect the proportion of Tim-3^＋PD-1^＋, Tim-3^-PD-1-, Tim-3^-PD-1^＋ and Tim-3^＋PD-1^- in CD3 T cells, and the expression of interferon （IFN）-γ and interleukin （IL）-10 produced by the four parts of T cells, from 17 normal pregnant women and 19 RSA patients.ResultsCompared with normal pregnant women group （1.24%±0.77%）, the proportion of Tim-3^＋PD-1^＋T cells in RSA group were lower significantly （0.57%±0.26%）（P〈0.001）. The proportions of IL-10 in four parts of T cells in RSA group were 33.55%±16.27%, 0.92%±0.88%, 1.61%±1.35% and 16.36%±13.98%. The proportions of IL-10 in four parts of T cells in normal pregnant group were 45.92%±17.89%, 0.49%±0.27%, 0.92%±0.68% and 33.43%±16.98%. The proportion of IL-10 produced by Tim-3^＋PD-1^＋ T cells in two groups was higher than that in other three parts （P〈0.05）; and the proportions of IL-10 produced by Tim-3^＋PD-1^＋ and Tim-3^＋PD-1-T cells in RSA group （33.55%±16.27%, 16.36%±13.98%） were lower than those in normal pregnant group （45.92%±17.89%, 33.43%±16.98%） （P〈0.05, P〈0.01）, while the expression of IFN-γ in four parts of two groups had no difference.ConclusionIL-10＋Tim-3^＋T cells are significantly lower in RSA, which may be a new observation index for judging the RSA.