摘要
目的:探讨尿酸对类成骨细胞(MG-63)增殖的影响及其可能机制。方法:将生长状态良好的类成骨细胞(MG-63)分为四组,分别为对照组(加入成骨培养液的完全培养基)和实验组(分别加入成骨培养液及含0.2、0.4、0.8mmol/L尿酸的完全培养基),诱导第14天,在倒置显微镜下观察细胞形态变化,分别在第7天和第14天检测类成骨细胞(MG-63)碱性磷酸酶活性,CCK-8法检测细胞增殖情况以及RT-PCR法检测TGF-β1 mRNA的表达。结果:尿酸干预类成骨细胞(MG-63)后,细胞数目随着尿酸浓度的升高逐渐增加,以0.8 mmol/L最明显。类成骨细胞(MG-63)碱性磷酸酶活性与增殖能力均增高;细胞TGF-β1 mRNA表达升高,呈现浓度、时间依赖性;以上指标于实验组与对照组间以及各实验组组间比较,差异均有统计学意义(P<0.05)。结论:尿酸可刺激类成骨细胞(MG-63)增殖,可能与促进TGF-β1转录有关。
Objective: To investigate the effect and mechanism of uric acid (UA) on the proliferation of osteoblast-like cells (MG-63) in vitro. Methods: The osteoblast-like cells (MG - 63) were divided into four groups: control group treated by osteoblast inducing media in complete medium and treatment groups by treated by osteoblast inducing media and different concentrations of uric acid (0.2 mmol/L, 0.4 mmol/L, 0.8 mmol/L) in complete medium. After fourteen days of induction, the cell morphology was observed under an inverted microscope. After seven and fourteen days of induction, the cell proliferation was identified by alkaline phosphatase activity and cell counting kit-8 (CCK-8) method. The expression of transforming growth factor-β1 (TGF -β1) mRNA was detected by reverse transcription PCR (RT-PCR). Results: Cells was increased more with the group of higher contentions ofucid acid, and the cells in 0.8mmo/L uric acid formed the most numbers among all the groups. Cell proliferated capacity and the alkaline phosphatase of each group with different concentrations of uric acid interfering the MG - 63 were increased, the expression of TGF-β1 mRNA was evaluated with the increase of UA concentrations and with intervention time. The comparison between each experimental group and control group or the comparison between each experimental group has statistic significance (p〈 0.05). Conclusions: The uric acid could promote the proliferation of osteoblast-like cells (MG - 63), which might he associated to promote the transcription of TGF-betal.
作者
王淑芳
杨乃龙
孙玉英
谢婷婷
WANG Shu-fang YANG Nai-long SUN Yu-ying XIE Ting-ting(Department of Endocrnology, Affiliated Hospital of Qingdao University, Qingdao, Shandong, 266000, China Caoxian People's Hospital, Heze, Shandong, 274400, China)
出处
《现代生物医学进展》
CAS
2017年第12期2227-2231,2240,共6页
Progress in Modern Biomedicine
基金
国家自然科学基金项目(30871192)
青岛市科技基金项目(08-2-1-5-nsh-6)