湖北省黄冈地区啮齿动物携带汉坦病毒M片段基因特征分析

Genetic analysis of M segment in hantaviruses carried by wild rodents from the Huanggang area,Hubei province

目的:探讨湖北省黄冈地区野生啮齿动物自然感染汉坦病毒(hantavirus)情况及病毒基因分型特征.方法:2012~2013年秋冬季在湖北省黄冈地区的野外及居民区采用夹夜法捕鼠.对捕获的动物进行分类鉴定,取其肺脏提取RNA,合成Seoul病毒(SEOV)和汉滩病毒(HTNV)特异性引物,以RT-PCR方法扩增部分M片段核苷酸序列,对PCR产物进行测序,并进行系统发育和序列同源性分析.结果:共捕获啮齿类动物95只,其中褐家鼠75只(78.94%).6份(8.00%)褐家鼠标本SEOV阳性.系统发育分析关系显示当地SEOV属于A组,与Z37株同源性为95.80%~96.30%(核苷酸),97.90%~100%(氨基酸).黄胸鼠和黑线姬鼠SEOV和HTNV均为阴性.结论:湖北省黄冈地区的优势鼠种是褐家鼠,汉坦病毒型别主要是SEOV,其系统发育关系属于A组,且与疫苗株Z37同源性最高.该研究结果有助于为黄冈地区HFRS的预防提供理论依据.

AIM： To study the infection and genotyping charac- teristics of hantavirus in wild rodents from the Huanggang area in Hubei province, China. METHODS： Rodents were captured in residential area and fields with night trapping method in Huang- gang, Hubei province during autumn and winter from 2012 to 2013. The species of rodents were identified. Then RNA was extracted from lung tissues. Partial M segments of Seoul virus （SEOV） and hantaan virus （HTNV） were detected by using specific RT-PCR. PCR products were sequenced. Phylogenetic relationship and sequence homology were analyzed by using Mr- Bayes 3.1 and BioEdit. RESULTS： A total of 95 rodents were captured, including 75 （78.94%） Rattus norvegicus. Six （80%） Rattus norvegicus were identified to be SEOV positive. Phyloge- netic analysis revealed that the SEOV in Huanggang belongs to the phylogroup A. The nucleotide sequence identifies between SEOV in Huanggang and stain Z37 were 95.80% to 96.30%, and amino acid sequence identities were 97.90% to 100%. Rattus flavipectus and Apodemus agrarius were both identified to be SEOV negative and HTNV negative. CONCLUSION： The dominant rodent spe- cies was Rattus norvegicus in Huanggang, Hubei province. The predominant hantaviruses carried by these rodents were SEOV, which belonged to phylogroup A, and were closely related with strain Z37. This research is helpful for the prevention of HFRS in Huanggang area, Hubei province, China.