黄酮类成分在蜂蜜抗菌性中的效能研究

Study on the Efficacy of Flavonoids in the Antibacterial Properties of Honey

目的为了解蜂蜜的抗菌性与其黄酮类成分含量的相关性。方法采用UV测定蜂蜜中的总黄酮含量;采用UPLC-MS/MS,以12种黄酮类成分为对照品对蜂蜜中的黄酮类成分及其含量进行测定;以平皿法检测蜂蜜和81%糖溶液在不同质量分数下的抗菌性能。结果 7个市售蜂蜜均含有黄酮类物质,但因蜜源植物不同,总黄酮含量差异显著,其中以枣花蜜(千红)含量最高(87.138μg·g^(-1)),玫瑰花蜜(青海)含量最低(28.649μg·g^(-1)),12种黄酮类成分在各蜂蜜中的含量各不相同,其中以白杨素在总黄酮中含量占比最高,最高占比达85.1%,其次为生松素、山奈酚和芹菜素;供试蜂蜜和81%糖溶液均表现出一定的抗菌性,但抗菌效果因蜂蜜品种而异,其中总黄酮含量越高的蜂蜜,其抗菌性表现也较好,81%糖溶液的高渗性对黑曲霉和白色念珠菌不起作用。结论黄酮类组分含量与蜂蜜的抗菌性呈一定程度的正相关,蜂蜜的抗菌性能可用于进一步鉴别蜂蜜的真假。

OBJECTIVE To understand the relationship between the antibacterial activity of honey and the content of flavonoids. METHODS UV method was used to determine the content of total flavonoids in honey, and UPLC-MS/MS method was used to determine the content of flavonoids by 12 kinds of flavonoids as control. The antibacterial properties of honey in different mass fractions was detected by using the method of plate method. RESULTS Seven commercial honey contain flavonoids, but due to the different nectar plants, there was significant difference in the content of total flavonoids, of which the date honey（Qianhong） content was the highest（87.138 μg·g^-1）, rose honey（Qinghai） was the lowest（28.649 μg·g^-1）. The contents of 12 kinds of flavonoids in different honey were different. The proportion of chrysin in total flavonoids content was the highest（85.1%）, followed by pinocembrin kaempferol and apigenin. All the samples showed a certain antibacterial activity, but the antibacterial effect was different with the varieties of honey, and the higher total flavonoids content, the better antibacterial activity. CONCLUSION The content of flavonoids and the antibacterial activity of honey are positively related to some degree, antibacterial properties of honey can be used for further identification of genuine and supposititious honey.