HPLC测定紫杉醇纳米脂质体凝胶剂在小鼠皮肤中的含量

Determination of Paclitaxel Nano-liposomes Gels Content in Mouse Skin by HPLC

目的考察紫杉醇纳米脂质体凝胶剂给药4 h后在小鼠皮肤各层中的含量分布情况。方法采用胶带粘贴技术剥脱角质层,以HPLC测定紫杉醇在皮肤各层中的含量,比较紫杉醇脂质体凝胶剂以及紫杉醇凝胶剂在皮肤各层中的分布特点。结果紫杉醇纳米脂质体给药4 h后在角质层和活性皮肤层中的药物含量分别为3.41,5.35μg·cm-2,与紫杉醇凝胶剂比较,能显著提高药物在皮肤中的滞留量(P<0.05)。结论与紫杉醇凝胶剂相比,紫杉醇纳米脂质体能增加药物的皮肤滞留量。

OBJECTIVE To investigate the distribution of paclitaxel content in mouse skin after 4 h topical application of paclitaxel nano-liposomes gels. METHODS Mouse superficial skin samples were obtained by tape stripping technique and paclitaxel content in the stratum corneum and viable skin was determined by HPLC after extraction. RESULTS The paclitaxel content in the stratum corneum and viable skin were 3.41, 5.35 μg·cm^-2 respectively after 4 h topical application of paclitaxel nano-liposomes gels. CONCLUSION Compared with paclitaxel gels, nano-liposomes gels can significantly improve the skin residual amount.