一测多评法同时测定妇乐颗粒中4种成分的含量

Simultaneous Determination of 4 Constituents in Fule Granula by Quantitative Analysis of Multi-components by Single Marker(QAMS)

目的采用一测多评法同时测定妇乐颗粒中绿原酸、大黄素、大黄酚与延胡索乙素的含量。方法采用Agilent ZORBAX Eclipse XDB C18色谱柱(4.6 mm×250 mm,5μm),流动相为乙腈(A)-0.2%磷酸水溶液(B),梯度洗脱:0~8.0 min,85%B;8.0~15.0 min,85%→45%B;15.0~30.0 min,45%→20%B;分段变波长测定:0~8.0 min为327 nm,8.0~15.0 min为254 nm,15.0~30.0 min为280 nm;流速为1.0 m L·min^(-1);柱温为30℃。测定大黄素与绿原酸、大黄酚和延胡索乙素间的相对校正因子,考察其重复性,比较计算值与测得值的差异。结果用一测多评法对6批妇乐颗粒中绿原酸、大黄素、大黄酚与延胡索乙素进行测定,与外标法实测值基本一致。结论该方法可靠,结果准确,可用于妇乐颗粒的质量控制。

OBJECTIVE To develop a method of quantitative analysis of multi-components by single marker（QAMS） for determination of chlorogenic acid, emodin, chrysophanol, dl-tetrahydropalmatine in Fule granula. METHODS The Agilent ZORBAX Eclipse XDB C18（4.6 mm×250 mm, 5 μm） column was used; mobile phase was acetonitrile（A）-0.2% phosphoric acid solution（B）. Gradient elution： 0-8.0 min, 85% B; 8.0-15.0 min, 85%→45% B; and 15.0-30.0 min, 45%→20% B. Wavelengths： 0-8.0 min was 327 nm, 8.0-15.0 min was 254 nm and 15.0-30.0 min was 280 nm. Flow rate was 1.0 m L·min^-1 with column temperature 30 ℃. Relative correction factors of chlorogenic acid, chrysophanol and tetrahydropalmatine were determined with emodin as internal standard. The method was evaluated for reproducibility, and the difference between calculated and measured values was compared. RESULTS No significant differences were found in the quantitative results of chlorogenic acid, emodin, chrysophanol, dl-tetrahydropalmatine determined by using QAMS and external standard method. CONCLUSION The QAMS method was reliable and accurate, which might be used for the quality control of Fule granula.