HPLC同时测定小蓟配方颗粒中蒙花苷和芹菜素的含量

Determination of Linarin and Apigenin in Cirsii Herba Dispensing Granule by HPLC

目的建立HPLC同时测定小蓟配方颗粒中蒙花苷和芹菜素含量的方法。方法采用Diamonsil-C_(18)(4.6 mm×250 mm,5μm)色谱柱,以乙腈-1%冰醋酸水溶液为流动相进行梯度洗脱,流速:1.0 m L·min^(-1),检测波长:335 nm,柱温:30℃。结果蒙花苷和芹菜素进样量分别在0.3~1.5μg(r=0.999 4)和0.075~0.375μg(r=0.999 7)内与峰面积呈良好的线性关系;加样回收率分别为99.19%和99.12%,RSD分别为1.27%和1.26%。结论该方法操作简便,重复性好,可用于小蓟配方颗粒中蒙花苷和芹菜素的含量测定。

OBJECTIVE To develop a method for content determination of linarin and apigenin in Cirsii Herba Dispensing Granule by HPLC. METHODS The separation was carried out on Diamonsil-C（18） column（4.6 mm×250 mm,5 μm） under gradient elution with acetonitrile-1% glacial acetic as mobile phase at a flow rate of 1.0 m L·min^-1. The detection wavelength was 335 nm and the column temperature was maintained at 30 ℃. RESULTS There were good linear relationships between the masses and the peak areas of linarin and apigenin in the ranges of 0.3-1.5 μg（r=0.999 4） and 0.075-0.375 μg（r=0.999 7）, respectively. The average recoveries were 99.19% and 99.12%, and RSDs were 1.27% and 1.26%. CONCLUSION The method is simple and reliable for the determination of linarin and apigenin in Cirsii Herba Dispensing Granule.