开发高灵敏度血痕发光试剂用于法医现场检测

The application of a high-performance bloodstain testing reagent in forensic scene

目的开发高灵敏度血痕发光试剂用于法医现场检测。方法本项目通过借鉴ECL发光的原理,通过化学电子链的乒乓共轭激活原理,使用均匀设计实验方法优化条件,合成多个新化合物,开发出新型高灵敏度血痕发光试剂。结果本项目开发的高灵敏度血痕发光试剂灵敏度高,发光时间长,在血液稀释1000倍的情况下,使用Chemi Scope3300化学发光成像系统读取荧光值,经过灰度转化最高值达到56,发光时间达到10分钟。结论本项目成功开发了高灵敏度血痕发光试剂,可以应用于法医现场检测。

Objective To develop a highly sensitive luminescent reagent for bloodstain testing at forensiccrime scenes. Methods Based ontheprincipleof ECL luminescence and the ping-pong conjugate activation principleof chemical electronic chain, this project developed a new type of highly sensitive luminescent reagent forbloodstain testing by usingthe uniform design of experimental methods to optimize the conditions andsynthesizeseveral new compounds. Results The bloodstain testing luminescent reagentdeveloped in this project has highsensitivity andlongluminescence time. In the case of blood samples diluted by 1,000 times, reading the fluorescencewithChemiScope 3300 chemiluminescence imaging system , the maximumvalue of gray scale reached 56,andthe luminescence time lasted for 10 minutes. Conclusion The project has successfully developed a highly sensitivebloodstaintesting reagentthat could be applied to crime scene investigation.