高效液相色谱法测定大鼠血浆中西咪替丁的浓度

Determination of Cimetidine in Rat Plasma by HPLC

目的建立一种高效液相色谱法(HPLC)检测大鼠血浆中西咪替丁的浓度。方法采用BDS HYPERSIL C18(5μm,4.6×250mm)色谱柱,流动相为甲醇-水-二乙胺-磷酸(25:75:0.47:0.38);流速:1m L·min-1;检测波长:225nm;进样量20μL;柱温30℃。结果西咪替丁在1μg·mL^(-1)~100μg·mL^(-1)浓度范围内与峰面积呈良好线性关系(r=0.9999);样品平均回收率为94%~104%,日内、日间RSD均低于5%(n=5)。结论本法专属性好,灵敏度高,操作简便,可用于大鼠血浆中西咪替丁含量的测定。

OBJECTIVE To establish an HPLC method for determination of cimetidine in rat plas- ma. METHODS The sample was analyzed in column BDS HYPERSIL C 18 （5μm,4. 6 × 250mm） ;mobile phase consisted of methonal-water-diethylamine-phosphate（25：75：0. 47： 0. 38 ） ;The flow rate was 1.0mL ·min-1;The de- tective wavelength was 225nm. The sample size was 20μL. The detection temperature was at 30℃. RESULTS The calibration curves of cimetidine showed good linerarity ,in the range of 11μg · mL-1 - 100μg · mL-l （ r = 0. 9999 ） ; The average recoveries of sample were in the range of 94% -104%. the intra-day and inter-day variation（RSD） was less than 5% （ n = 5 ）. CONCLUSION This method is accurate, simple, quick and with good sensitivity for determi- ning the cimetidine concentration in rat plasma.