川渝地区部分猪场猪瘟、猪伪狂犬病和口蹄疫的抗体监测

Antibody Surveillance on Classical Swine Fever,Pseudorabies and Foot-and-mouth Disease in Some Swine Farms of Sichuan-Chongqing Region

为评价猪场的疫苗免疫效果,制定合理的免疫程序。利用酶联免疫吸附(ELISA)的方法,对四川省、重庆市6个规模化养猪场的409份血清,分别进行猪瘟、猪伪狂犬病和口蹄疫的抗体检测。结果显示,A、B、C、D、E和F等6个猪场的猪瘟抗体阳性率分别为91%、85.71%、92.5%、79.8%、75%和97.33%,猪伪狂犬病g B抗体阳性率分别为100%、80.2%、100%、93%、75%和96%,猪口蹄疫抗体阳性率分别为72%、76.7%、79.59%、80.8%、0和82.67%,场间差异显著。统计学分析显示,母猪中的这3种疫病抗体阳性率均显著高于仔猪。其中,母猪、仔猪的猪瘟抗体阳性率分别为98.21%、27.27%,猪伪狂犬病g B抗体阳性率分别为98.21%、42.5%,猪口蹄疫抗体阳性率分别为83.41%、42.5%。本研究结果可为川渝地区规模化猪场疫苗免疫程序的制定提供参考。

In order to recognize the immune status and to establish proper immune program, levels of specific antibodies against Classical swine fever virus （CSFV）, Pseudorabies virus （PRV） and Foot-and-mouth disease virus （FMDV） in some farms of Sichuan-Chongqing region were investigated. By the method of ELISA, 409 serum samples from 6 large scale farms were conducted detection. Results showed that the antibody positive rates of CSFV in these six farms were 91%, 85.71%, 92.5%, 79.8%, 75% and 97.33%, that of PRV gB were 100%, 80.2%, 100%, 93%, 75%and96%, and thatofFMDVwere72%, 76.7%, 79.59%, 80.8%, 0and82.67%, respectively, showing significant difference. Furthermore, through statistical analysis, antibody levels against the 3 viruses existing in sows were all higher than those in piglets. The antibody positive rates of CSF in sows and piglets were 98.21% and 27.27%. And the corresponding numerical values were 98.21% and 42.5% in PRV, 83.41% and 42.5% in FMD. These findings would offer references for determining the immunization procedure for large-scale swine farms in Sichuan- Chongqing region.