江西省种猪群伪狂犬病血清学横断面研究

A Cross-sectional Study on Sero-prevalence of Pseudorabies in Swine Breeding Herds in Jiangxi Province

为了解江西省种猪群伪狂犬病(PR)的流行情况,寻找狂犬病毒(PRV)感染的潜在风险因素,开展了本次横断面研究。按照估计流行率的抽样策略,随机抽取73个种猪场,根据猪场养殖规模将其分为3种类型,分别进行抽样,然后采用PRV g E-ELISA抗体检测试剂盒进行抗体检测。同时,根据73个种猪场的详细地址,描绘猪场的空间分布图,并对其开展问卷调查,对问卷中涉及的风险因素进行单因素分析,筛选出P<0.2的变量,将其导入多因素Logistic回归模型,用倒推法选择变量,进行回归分析。结果发现:江西省种猪群PR的群流行率为28.63%(95%CI:18.26%~38.99%),个体流行率为9.37%(95%CI:8.98%~9.77%);PR阳性猪场多分布于江西省中部的上饶、抚州、鹰潭、宜春等县市;与PR感染相关的2个风险因素是场区周围3 km范围内是否有其他猪场(OR=4.766,P=0.020)和养殖场主是否愿意开展PR净化(OR=0.054,P=0.014)。依据研究结果,建议加强对养殖场主的培训与宣传,使其认识到PR净化的重要性,并掌握PR防控与净化的专业技术。

In order to estimate the prevalence of Pseudorabies（PR）in swine breeding herds in Jiangxi Province,and to identify the potential risk factors,a cross-sectional study was conducted. According to the sampling strategy,73 swine breeding farms were randomly selected and divided into 3 types based on their scales,then the selected serum samples were tested by PRV g E-ELISA kits. Meanwhile,a spatial distribution map was drawn in terms of the 73 detailed addresses,and a questionnaire survey was conducted to analyze the relevant risk factors. Firstly,variates screened from single factor analysis（P 0.2）were imported into multiple logistic regression model. Then by means of backward induction,the variates were chosen to carry out regression analysis. Results showed that in Jiangxi Province,the herd prevalence of PR was 28.63%（95%CI：18.26%～38.99%）,while the rate at individual level was 9.37%（95%CI：8.98%～9.77%）. Besides,the infected farms distributed mainly in the middle area of Jiangxi province,including Shangrao,Fuzhou,Yingtan and Yichun. Two risk factors associated PR infection were found,one was whether there were other swine farms within 3 km（OR=4.766,P=0.020）,and the other was if the farmers had willing to eradicate PR（OR=0.054,P=0.014）.This study indicated that further training and propaganda about PR eradication for farmers should be strengthened,which would conducive to let them realize the importance and master professional techniques for PR prevention and control.