老年晚期肺癌高凝状态患者外周血VEGF、MMP-9的表达

Expression of Serum VEGF,MMP-9 in Eldly Patients with Advanced NSCLC in Hypercoagulative State

目的研究老年晚期高凝状态肺癌患者外周血中血管内皮生长因子(VEGF)与基质金属蛋白酶-9(MMP-9)的表达情况.方法采用ELISA法检测VEGF、MMP-9在30例老年晚期高凝状态肺癌和30例无高凝状态的老年晚期肺癌患者血清中的表达水平.结果老年晚期高凝状态肺癌患者外周血VEGF、MMP-9的表达水平分别为(536±103)pg/m L、(474±76)ng/m L,均明显高于无高凝状态的老年晚期肺癌(433±74)pg/m L、(362±43)ng/m L,(P<0.05),且在老年晚期高凝状态肺癌外周血中VEGF水平与MMP-9的表达存在正相关关系(r=0.478),P<0.05,并其高表达与患者的年龄、临床分期及伴发疾病的数量相关(P<0.05).结论 VEGF、MMP-9在老年晚期高凝状态肺癌外周血中高表达,进一步证实血液高凝状态在一定程度上促进老年晚期肺癌的侵袭转移.

Objective To investigate the expression and significance of serum VEGF （vascular endothelial growth factor） and MMP-9 （matrix metalloprotoinase-9） in eldly patients with advanced NSCLC （non-small cell lung cancer） in hypercoagulative state. Methods Serum VEGF and MMP-9 were detected separately by ELISA （enzyme-linked immunosorbent assay） in 30 specimens of the eldly advanced NSCLC patients with hypercoagulative state and without hypercoagulative state. Results The expression level serum VEGF and MMP-9 was 536 ± 103 pg/mL and 474 ± 76 ng/mL in the eldly advanced NSCLC patients with hypercoagulative state; which were higher than that in without hypercoagulative samples （433 ± 74 pg/mL and 362 ± 43 ng/mL） （P〈0.05）. The higher expression of serum VEGF in the elder advanced NSCLC patients with hypercoagulative was positively correlated with the higher expression of MMP-9 （r=0.478） P〈0.05, and which was correlated with the age, the clinical TNM stage and the number of accompanying disease （P〈0.05）. Conclusion VEGF and MMP-9 are highly expressed in the eldly advanced NSCLC patients in hypercoagulative state, further confirming that hypercoagulative states to some extent may promote the invasion and metastasis of elderly patients with advanced lung cancer.