丹参酮ⅡA对肝癌细胞增殖凋亡作用及相关机制的研究

Effect of Tanshinone ⅡA on proliferation apoptosis of human hepatocellular carcinoma cell and research on related mechanism

目的:研究丹参酮ⅡA对肝癌HepG2细胞的增殖和凋亡的影响,并探讨相关的下游机制。方法:用梯度浓度(0μg/mL,12.5μg/mL,25μg/mL,50μg/mL,100μg/mL,200μg/mL)的丹参酮ⅡA作用于HepG2细胞,检测24 h,48 h对细胞的IC_(50)值。Tunel染色、流式细胞术检测不同加药组相对于HepG2细胞的凋亡情况,qPCR和WB实验检测p53相关基因m RNA和蛋白。结果:不同浓度的丹参酮ⅡA对HepG2细胞均有杀伤作用,并呈现剂量依赖性。在24 h和48 h的IC_(50)值分别为51.25μg/mL和47.36μg/mL。流式细胞术检测发现丹参酮ⅡA能诱导肝癌细胞的凋亡,并呈现一定的剂量依赖性。qPCR和WB实验则证实,丹参酮ⅡA作用细胞后,p53的表达量明显增加。结论:丹参酮ⅡA可以提高HepG2细胞中p53的表达,诱导细胞凋亡。

This article studies the effects of tanshinone Ⅱ A on proliferation and apoptosis of human hepatocellular carcinoma cell line HepG2 and explores the related underlying mechanism. The concentration of tanshinone Ⅱ A （0 μg/mL, 12.5 μg/mL 25 μg/mL, 50 μg/mL, HepG2, 100μg/mL, μg/mL, 200 μg/mL） was treated on HepG2 cells to detect the IC50 value at 24 h and 48 h. Tunel staining and flow cytometry are used to detect the effect of different dosing group of tanshinone Ⅱ A to detect the apoptosis of HepG2 cells, qPCR and WB are used to detect the mRNA and protein of p53 gene. This article finds that tanshinone Ⅱ A with different concentrations has a killing effect on HepG2 cells and shows dose-dependent manner. The IC50 values of 24 h and 48 h are 51.25 μg/mL and 47.36 μg/mL, respectively. Flow cytometry shows that tanshinone Ⅱ A could induce the apoptosis of hepatocellular carcinoma cells, and shows a dose-dependent manner. The results of qPCR and WB show that the expression of p53 is significantly increased after being treated with tanshinone 1T A. Therefore, the article comes to a conclusion that tanshinone Ⅱ A can increase the expression of p53 in HepG2 cells and induce its apoptosis.