泛素连接酶TRIM46促进人结直肠癌细胞增殖和侵袭

Ubiquitin ligase TRIM46 promotes the proliferation and invasion of colorectal cancer cells

目的:探讨siRNA下调三重基序(tripartite motif,TRIM)46蛋白表达对结直肠癌HCT116细胞增殖和侵袭功能的影响及可能的作用机制。方法:将TRIM46 siRNA转染至人结直肠癌HCT116细胞后,应用实时荧光定量PCR和蛋白质印迹法检测细胞中TRIM46 mRNA及蛋白的表达水平,CCK-8法和Transwell法分别检测下调TRIM46表达对HCT116细胞增殖和侵袭能力的影响,蛋白质印迹法检测细胞中SH2结构域酪氨酸磷酸酶1[Src homology 2(SH2)-containing tyrosine phosphatase 1,SHP1)]蛋白的表达。结果:TRIM46 siRNA转染后,HCT116细胞中TRIM46 mRNA及蛋白表达水平被显著抑制(P值均<0.05)。下调TRIM46表达可显著抑制结直肠癌HCT116细胞的增殖和侵袭(P值均<0.05),而SHP1蛋白表达水平明显升高(P<0.05)。结论:泛素连接酶TRIM46可能通过抑制SHP1表达,促进结直肠癌细胞的增殖和侵袭。

Objective： To explore the effect of tripartite motif 46 （TRIM46） on the proliferation and invasion of colorectal cancer cells and study its possible mechanism.Methods： After TRIM46 siRNA was transfected into human colorectal cancer HCT116 cells, the expressions of TRIM46 mRNA and protein were detected by real-time fluorescent quantitative PCR and Western blotting, respectively. CCK-8 assay and Transwell chamber assay were implemented to observe the effect of TRIM46 down-regulation on the proliferation and invasion of HCT116 cells, and the expression of Src homology 2 （SH2）-containing tyrosine phosphatase 1 （SHP1） was detected by Western blotting.Results： After TRIM46 siRNA was transfected into human colorectal cancer HCT116 cells, the expression levels of TRIM46 mRNA and protein were inhibited （both P 〈 0.05）. Knockdown of TRIM46 significantly suppressed the cell proliferation and invasion of HCT116 cells （both P 〈 0.05）. However, the expression level of SHP1 was increased （P 〈 0.05）.Conclusion： Ubiquitin ligase TRIM46 may improve the proliferation and invasion of human colorectal cancer cells through suppressing SHP1.