猪伪狂犬病基因缺失灭活疫苗(JS-2012-△gI/gE株)的制备及免疫效力评价

PREPARATION AND IMMUNE EFFICACY EVALUATION OF INACTIVATED SWINE PSEUDORABIES VACCINE(JS-2012-△gI/gE STRAIN)

本研究将猪伪狂犬病毒双基因缺失毒株（JS-2012-△g I/g E株）用0.15%的甲醛灭活后,与赛彼科公司MONTANIDE ISA201VG佐剂进行乳化,制备成猪伪狂犬病灭活疫苗。为了评价灭活疫苗的免疫效力,15头14日龄的健康仔猪（猪伪狂犬病毒、猪繁殖与呼吸综合征病毒、猪瘟病毒和猪圆环病毒Ⅱ型抗原和抗体均为阴性）随机分成3组,每组5头。第一组猪颈部肌肉接种猪伪狂犬病灭活疫苗（JS-2012-△g I/g E株）每头2 m L,接种后28 d,第一组和第二组同样方式同等剂量接种猪伪狂犬病灭活疫苗（JS-2012-△g I/g E株）,第三组猪作为对照。第二次免疫后d 28,三组实验猪均滴鼻接种PRV JS-2012第5代强毒,每头105.0TCID50/2 m L。攻毒后0~14 d,每日测量体温并记录临床症状。通过抗体检测和保护效率来评价猪伪狂犬病灭活疫苗（JS-2012-△g I/g E株）免疫效力。结果表明,两次免疫（第一组）PRV g B抗体水平明显高于一次免疫（第二组）,两次免疫提供了更好的保护效力。

The Pseudorabies virus （PRV, JS-2012-△gl/gE strain） inactivated with 0.15% formalin was homogenized with MONTANIDE ISA 201 VG （SEPPIC） to prepare the inactivated swine PR vaccine. In order to evaluate the protective efficacy of the inactivated swine PR vaccine, fifteen 2-week-old piglets which were free of PRV, porcine reproductive and respiratory syndrome virus （PRRSV）, porcine circovirus （PCV2） and classical swine fever virus （CSFV）） were selected randomly and divided equally into three groups. Group 1 were inoculated intramuscularly （i.m.） with 2 mL of inactivated swine Pseudorabies vaccines （JS-2012-△gl/gE strain）. At 28 days- post-first inoculation, group 1 and group 2 were inoculated intramuscularly, with the inactivated vaccines at the same dosage, group 3 served as uninoculated control. At 28 days post-inoculation （DPI）, all piglets were challenged intranasally with 10^5.0 TCID50 of PRV （JS- 2012 strain）. Following the challenge, clinical signs and rectal temperatures were recorded daily for 14 days. The results showed PRV g B antibody levels of piglets vaccinated twice （Group 1） was higher than those vaccinated once （Group 2）, The protective efficacy of the inactivated swine PR vaccine against PRV after double inoculation（Group 1） was better than that after single inoculation （Group 2）.