重楼皂苷I对体外抑制冠状动脉内皮细胞增殖的影响

Effect of Saponins I Extracted from Paris Polyphylla on Coronary Artery Endothelial Cell Proliferation

目的观察重楼皂苷I对冠状动脉内皮细胞增殖的影响.方法重楼皂苷I处理冠状动脉内皮细胞后,通过细胞计数法计算冠状动脉内皮细胞的生长速度,MTT法测定冠状动脉内皮细胞活性,RT-PCR测定钙粘蛋白和Caspase3mRNA表达.结果重楼皂苷I减慢冠状动脉内皮细胞的生长速度,降低冠状动脉内皮细胞活力,降低冠状动脉内皮细胞的钙粘蛋白mRNA表达,增加Caspase3 mRNA表达.结论重楼皂苷I抑制冠状动脉内皮细胞增殖,诱导冠状动脉内皮细胞凋亡.

Objective To explore the effect of ParidissaponinI （PSI） on the proliferation of coronary artery endothelial cells （CAECs） . Methods CAECs were cultured with PSI. CAEC growth rate was calculated by using blood cell counting plate. Cell viability was measured by MTT. The expressions of cadherin and caspase3 mRNA were detected by RT-PCR. Results PSI slowed down growth rate of CAECs, reduced cell viability of CAECs, decreased the expression of cadherln mRNA and increased the expression of caspase3 mRNA in CAECs. Conclusion PSI inhibits the proliferation of CAECs and induces CAECs apoptosis.