三七皂苷Rg1对慢性应激大鼠脑组织神经颗粒素表达的影响

Research of Ginsensode Rg1 on the Expression of Neurogranin and Behavioral Alteration in Chronic Unpredictable Stress Model Rats

目的探讨三七皂苷Rg1对慢性应激模型大鼠皮层、海马神经颗粒素(neurogranin,Ng)表达的影响.方法成年雄性SD大鼠36只,随机分为对照组(CON)、模型组(CUS)和治疗组(CUS-G),采用慢性不可预见性应激方法建立慢性应激动物模型,运用Morris水迷宫实验对大鼠进行学习记忆力测试,QPCR、Western blot分别检测皮层、海马Ng m RNA及Ng蛋白含量.结果水迷宫实验显示慢性应激后动物学习记忆能力显著降低,而治疗组大鼠逃避潜伏期明显降低(P<0.0 5);应激6周后,慢性应激大鼠皮层、海马Ng m RNA水平皆与模型大鼠有差异(P<0.05、P<0.01),治疗组大鼠皮层、海马Ng m RNA水平皆与模型大鼠有差异(P<0.01、P<0.05);慢性应激大鼠皮层、海马Ng含量水平与模型大鼠有差异(P<0.05、P<0.01),治疗大鼠皮层、海马Ng含量亦与模型大鼠有差异(P<0.01、P<0.05).结论慢性不可预见性应激导致大鼠皮层、海马Ng m RNA水平、NG蛋白含量显著下降,造成大鼠学习记忆功能减退,三七皂苷Rg1可上调Ng m RNA水平、NG蛋白含量表达,且对慢性应激所致外显行为有积极调节作用.

Objective To investigate the effect of Ginsensode Rg1 on the expression of Neurogranin（Ng） and behavioral alteration in cortex and hippocampus of rats with chronic stress model. Methods A total of 36 adult male SD rats were randomly divided into control group（CON）, model group（CUS） and treatment group（CUS-G）. The chronic stress model was established by chronic unpredictable stress. The Morris water maze was used to study the learning and memory ability. The content of Ng in cortex, hippocampus was detected by RT-PCR and Western blot.Results The water maze test showed that after chronic stress, animal learning and memory ability decreased significantly, while the treatment group rats escape latency was significantly reduced（P〈0.05）;after 6 weeks of stress,the cortex and hippocampus Ng m RNA levelschronic stress rats were markedly lower than that of model rats respectively（P 〈0.05, P 〈0.01, P 〈0.05）. The cerebral cortex and hippocampus Ng m RNA levels in treatment group were significantly increased compared with that of model group respectively（P 〈0.01, P 〈0.05, P 〈0.05）; The cerebral cortex and hippocampus Ng levels of chronic stress rat were significantly decreased when compared with that of the model rats respectively（P〈0.05,P〈0.01,P〈0.05）,The cerebral cortex and hippocampus Ng content were significantly increased in treatment group compared with the model group respectively（P 〈0.01, P 〈0.05）. Conclusions Chronic stress can change the behaviors of mice in recognization and memory The contents of Ng and the supplement of Ginsensode Rg1 have positive adjustment.