云南白药对体外培养人牙周膜成纤维细胞C-fos、C-jun基因表达的影响

Effects of Yunnan BaiYao on the Expressions of c-fos and C-jan mRNA in Periodontal Ligament Cells in Vitro

目的研究云南白药水溶液对牙周膜成纤维细胞C-fos、C-jun基因表达的影响,探讨云南白药水溶液促进人牙周膜成纤维细胞（HPDLFs）增殖和成骨分化的可能机制.方法原代培养HPDLFs,取第4~6代细胞用于实验.分为空白对照组,云南不同浓度云南白药水溶液组,阳性对照组.各组细胞与云南白药水溶液共培养4h,采用反转录聚合酶链反应检测C-fos和C-jun m RNA的表达.结果 RT-PCR的结果说明,与空白对照组相比,云南白药水溶液能促进HPDLFs细胞C-fos和C-jun m RNA的表达.且促进作用呈浓度依赖性,与对照组比较,各组差异有统计学意义（P〈0.05）.结论云南白药水溶液能够上调HPDLFs中C-fos、C-jun的表达.云南白药水溶液可能通过上调C-fos、C-jun的表达促进HPDLFs的增殖和成骨分化,促进骨形成.

Objective To study the effects of Yunnan Bai Yao on the expressions of c-fos and c-jun m RNA in HPDLFs and explore the potential mechanism that Yunnan Bai Yao promotes the proliferation and differentiation of HPDLFs.Methods The HPDLFs tissue was obtained from the extracted healthy premolar.The HPDLFs used underwent four to six passages.Cells were divided into untreated group, positive control group and treated group. In treated group, HPDLFs were co-cultured with Yunnan Bai Yao solution for 4h with gradient concentration. The expressions of c-fos and c-jun m RNA were determined by RT-PCR.Results The results showed that the expressions of c-fos and c-jun m RNA were up-regulated significantly in treated group compared with control group（P〈0.05）.Conclusion Yunnna Bai Yao can upregulate the expressions of c-fos and C-jun m RNA in HPDLFs.Through upregulating the expressions of c-fos and c-jun m RNA in h PDLs,Yunnan Bai Yao can promote proliferation and differentiation of ossification of HPDLFs to induce bone formation.