人类男性29个Y-SNP位点种属特异性

Species Specificity of Male Human's 29 Y-SNP Loci

目的探讨人类男性29个Y-SNP位点的种属特异性,为法医学应用奠定基础.方法提取8种常见动物的DNA,用本研究设计的29个Y-SNP位点的引物进行PCR扩增,聚丙烯酰胺凝胶电泳、银染显色法进行基因分型.结果在分析的29个Y-SNP位点中,有23个位点只对人类男性DNA有扩增产物,有较好的种属特异性;有3个位点对人和部分动物有扩增产物,但产物的片段大小与人不同,3个位点对人和动物均有扩增产物,且片段长度与人类相近;5个位点对男性与女性样本均有扩增产物,且片段长度相等.结论在研究的29个Y-SNP位点中,大部分位点只对人类男性DNA有扩增产物,有较好的种属特异性,可用于法医学个人识别和亲子鉴定.

Objective To explore species specificity of 29 Y-SNP loci and to lay the foundation for forensic application. Methods Human DNA and 8 different kinds of common animals＇ DNA were amplified separately by PCR. The PCR products were analyzed with PAGE. Results Twenty-three out of 29 Y-SNP loci were only amplified by the male human DNA, which indicated a good species specificity. Three loci amplified from human and some animals showed that the length of the product was different between two species. Another three loci amplified from human and animal showed that the length of the fragment was similar between human and animal. Five loci were amplified by PCR products from both male and female samples. Conclusion Most of 29 Y-SNP loci, amplified in male humans, have good species specificity and can be directly used for personal identification and paternity testing.