微米大黄炭白芨胶对胃黏膜损伤的保护作用及机制

Micron rhubarb charcoal plus Bletilla striata gelatin protects against gastric mucosal injury in rats

目的研究微米大黄炭白芨胶调控局部黏着斑激酶(focal adhesion kinase,FAK)、Vinculin、kindlin-2对胃黏膜损伤的保护作用.方法选用80只SD大鼠,随机分为4组处理:对照组、模型组、微米大黄炭组、微米大黄炭白芨胶组.造模后第14天,检测各组大鼠黏膜损伤面积;放射免疫法检测各组大鼠血清中前列腺素E_2(prostaglandin E_2,PGE_2)、胃泌素(gastrin,GAS)含量及胃黏膜组织白介素-8(interleukin-8,IL-8)、肿瘤坏死因子-α(tumor necrosis factorα,TNF-α)含量;提取胃组织总RNA和总蛋白,RT-PCR及Western blot检测FAK、Vinculin、kindlin-2的mRNA及蛋白表达.结果与对照组相比,模型组大鼠的胃黏膜损伤面积显著增大(P<0.01),血清中PGE_2含量显著降低(P<0.01),GAS含量显著升高(P<0.01),胃黏膜组织中TNF-α、IL-8的含量显著升高(P<0.01),FAK、Vinculin、kindlin-2的mRNA及蛋白表达均显著下降(P<0.01);与模型组相比,微米大黄炭组与微米大黄炭白芨胶组大鼠胃黏膜损伤面积显著减少(P<0.01),血清中PGE_2含量显著升高(P<0.01),GAS含量显著降低(P<0.01),胃黏膜组织中TNF-α、IL-8的含量显著降低(P<0.01),FAK、Vinculin、kindlin-2的mRNA及蛋白表达均显著升高(P<0.01),微米大黄炭白芨胶组各检测指标变化均优于微米大黄炭组.结论微米大黄炭白芨胶能显著改善模型大鼠胃黏膜损伤状况,对损伤胃黏膜的保护作用可能与FAK、Vinculin、kindlin-2的表达增强有关.

AIM To investigate whether micron rhubarb charcoal plus Bletilla striata gelatin can protect against gastric mucosal injury by regulating the expression of focal adhesion kinase（FAK）,vinculin,and kindlin-2.METHODS Eighty SD rats were randomly divided into four groups：control group,model group,micron rhubarb charcoal group,micron rhubarb charcoal plus Bletilla striata gelatin group.After treatment for 14 days,the area of mucosal lesions was detected.Radioimmunoassay was used to detect the contents of prostaglandin E2（PGE2） and gastrin（GAS） in serum,and interleukin-8（IL-8） and tumor necrosis factor-α（TNF-α） in the gastric mucosa.RT-PCR and Western blot were used to detect the mRNA and protein expression of FAK,vinculin and kindlin-2.RESULTS Compared with the control group,the injury area in the gastric mucosa was significantly increased（P〈0.01）,the content of GAS in serum and the levels of TNF-α and IL-8 in the gastric mucosa（P〈0.01） were significantly increased,the content of PGE2 in serum and the mRNA and protein expression of FAK,vinculin,and kindlin-2 were significantly decreased in the model group（P〈0.01）.Compared with the model group,the area of gastric mucosal lesions was significantly decreased（P〈0.01）,the content of GAS in serum and the levels of TNF-α and IL-8 in the gastric mucosa were significantly decreased（P〈0.01）,the content of PGE2 in serum and the mRNA and protein expression of FAK,vinculin,and kindlin-2 were significantly increased in the two treatment groups（P〈0.01）.The changes in the above indexes were more significant in the micron rhubarb charcoal plus Bletilla striata gelatin group than in the micron rhubarb charcoal group.CONCLUSION Micron rhubarb charcoal plus Bletilla striata gelatin can significantly improve gastric mucosal injury in rats,and the protective effect may be related to un-regulating the expression of FAK,vinculin,and kindlin-2.