肝细胞肝癌中HDAC3的表达及临床意义

Clinical significance of expression of histone deacetylase 3 in hepatocellular carcinoma

目的探讨组蛋白去乙酰化酶3(histone deacetylase 3,HDAC3)在肝细胞癌(hepatocellular carcinoma,HCC)组织中的表达及其临床意义.方法采用免疫组织化学法检测60例HCC组织及癌旁组织石蜡切片中HDAC3的表达,分析其与HCC临床病理特征及与患者预后的关系.结果 HCC组织中HDAC3的表达水平明显高于其相应癌旁组织(P<0.05).HDAC3在HCC中的表达水平与HCC患者的性别、年龄、乙型肝炎病毒感染、TNM分期、病理分级、AFP水平、是否合并肝硬化、肿瘤数目、肿瘤大小和肿瘤结节的单发多发数目等无关(P>0.05),与肿瘤复发有相关性(P<0.05).HDAC3的表达与p-STAT3的表达呈正相关(r^2=0.622,P<0.001).HDAC3高表达组患者的术后生存时间明显低于低表达组患者(P<0.05),提示HDAC3高表达可能与HCC患者的预后有关.结论 HDAC3表达升高可能与HCC的发生、发展密切相关.

AIM To detect the expression of histone deacetylase 3（HDAC3） in hepatocellular carcinoma（HCC）and analyze its clinicopathological significance.METHODS Immunohistochemistry was performed in 60 pairs of HCC tissues and tumor-adjacent normal tissues.The relationship of HDAC3 expression with clinical and pathological features and overall survival was analyzed statistically.RESULTS HDAC3 expression was significantly upregulated in the HCC specimens compared to corresponding normal tissues（P〈0.05）.The expression of HDAC3 in HCC had no significant correlation with gender,age,history of hepatitis B virus infection,TNM stage,pathological classification,α-fetoprotein level,liver cirrhosis,tumor size,or tumor number（P〉0.05）.There was a positive correlation between HDAC3 and p-STAT3 expression in HCC tissues（r^2 = 0.622,P〈0.001）.However,HDAC3 expression had a significant correlation with tumor recurrence（P〈0.05）.The overall survival of postoperative HCC patients in the HDAC3 positive group was obviously poorer than that of patients in the HDAC3 negative group（P〈0.05）,suggesting that high HDAC3 expression is associated with a poor prognosis in HCC patients.CONCLUSION The up-regulated expression of HDAC3 may be closely related with the occurrence and development of HCC.