藁本内酯对H_2O_2诱导的B16黑素瘤细胞氧化损伤的保护作用

Protective Effect of Z-ligustilide on the Oxidation Damage of B16 Melanoma Cells induced by H_2O_2

目的:观察藁本内酯对H_2O_2诱导的B16黑素瘤细胞氧化损伤的保护作用并探讨其可能机制。方法:以H_2O_2诱导B16黑素瘤细胞氧化损伤为模型,并以藁本内酯进行干预,采用MTT法测细胞活力,酶标仪检测乳酸脱氢酶(LDH)漏出量,流式细胞术测细胞凋亡率、线粒体膜电位(△Ψm)和细胞内游离钙离子浓度。结果:与H_2O_2诱导的B16黑素瘤细胞比较,应用藁本内酯(5、10、20μmol·L^(-1))处理的B16黑素瘤细胞活力和△Ψm明显提高,LDH漏出量明显减少,细胞凋亡率和细胞内游离钙离子浓度明显降低,差异均具有统计学意义(P<0.05)。结论:藁本内酯对H_2O_2诱导的B16黑素瘤细胞氧化损伤具有保护作用,其作用机制可能通过恢复线粒体功能、抑制细胞凋亡有关。

Objective： To investigate the protective effects and possible mechanisms of Z-ligustilide（LIG） on the oxidation damage of B 16 melanoma cells induced by H2O2. Methods： The oxidative damage model of melanoma B 16 cells was induced by H2O2. And the model was treated by different concentration of LIG. The cell vitality was determined by MTT, leakage amount of lactate dehydrogenase （LDH） was detected by enzyme standard instrument. Cell apoptotic rate, mitochondrial membrane potential （△Ψm） and Ca^2＋concentra- tion in the cell were measured by flow cytometry. Results： Compared with model group, the cell vitality and △Ψm of LIG （5, 10, 20 μmol·L^-1） treated B16 melanoma cells were obviously improved, LDH leakage were significantly decreased, the apoptotic rate and Ca^2＋concentration were significantly decreased （P〈0.05）. Conclusions： LIG had protective effect on the oxidative damage of B16 melanoma cells induced by H2O2, which might be related to restoring the mitochondrial function, and the inhibition of the cell apoptosis.