Nesfatin-1对外侧臂旁核葡萄糖敏感性神经元兴奋性及大鼠摄食影响

EFFECTS OF NESFATIN-1 ON EXCITABILITY OF GLUCOSE-SENSITIVE NEURONS AND FEEDING IN LATERAL PARABRACHIAL NUCLEUS

目的观察Nesfatin-1对外侧臂旁核(LPBN)葡萄糖敏感性神经元兴奋性及大鼠摄食的影响,探究其调节摄食的可能机制。方法将Wistar大鼠随机分为Nesfatin-1组和生理盐水(NS)组。向单侧LPBN分别微量注射Nesfatin-1和NS,通过代谢笼监测大鼠夜间摄食量;通过在体电生理方法,向LPBN中神经元微压力注射葡萄糖,并鉴定是否为葡萄糖敏感性神经元。向LPBN中葡萄糖敏感性神经元微压力注射Nesfatin-1和NS,观察Nesfatin-1对LPBN葡萄糖敏感性神经元兴奋性的影响。结果 Nesfatin-1组6、12h累积摄食量与NS组相比明显降低(t=3.22、3.48,P<0.05),而两组1h和4h比较,差异无显著性(P>0.05)。LPBN存在葡萄糖敏感性神经元,且与NS组相比,Nesfatin-1可显著兴奋LPBN中的葡萄糖抑制性神经元(t=-4.99,P<0.01)。结论 LPBN注射Nesfatin-1可调节LPBN中葡萄糖敏感性神经元的兴奋性,并对大鼠夜间摄食有抑制作用。

Objective To observe the effects of Nesfatin-1on the excitability of glucose-sensitive neurons and feeding in the lateral parabrachial nucleus（LPBN）,and explore the possible mechanism of Nesfatin-1 on feeding regulation. Methods Wistar rats were randomly divided into Nesfatin-1group and normal saline（NS）group.Nocturnal food intake was measured by metabolic cage.Applying in vivo electrophysiological technique,the neurons were microinjection with glucose in LPBN to identify if they were glucose-sensitive neurons.Nesfatin-1and NS were microinjected into glucose-sensitive neurons in LPBN to observe the impact of Nesfatin-1and NS on neurons in LPBN. Results In Nesfatin-1group,the six-hour and 12-hour cumulative food intake was lower than that in NS group（t=3.22,3.48;P〈0.05）,and the difference of that in one-hour and four-hour food intake between the two groups was not significant（P〉0.05）.There were glucose-sensitive neurons in LPBN,compared with NS group,Nesfatin-1 could significantly excite the glucose-inhibitory neurons in the LPBN（t=-4.99,P〈0.01）. Conclusion Injection of Nesfatin-1into lateral parabrachial nucleus can modulate the excitability of glucose-sensitive neurons in the nucleus and inhibit the nocturnal feeding in rats.