口腔鳞状细胞癌病人人乳头状瘤病毒感染的检测

DETECTION OF HUMAN PAPILLOMAVIRUS INFECTION IN ORAL SQUAMOUS CELL CARCINOMA

目的探讨口腔鳞状细胞癌(OSCC)病人人乳头状瘤病毒(HPV)的感染情况。方法收集2014年7月—2016年7月我科收治的53例OSCC病人(实验组),取其肿瘤组织、癌旁组织及正常口腔黏膜组织标本。以我科收治的50例非OSCC病人及50例健康志愿者作为对照组,取其正常口腔黏膜组织标本。采用PCR-DNA反向点杂交HPV基因分型检测技术检测两组标本HPV感染情况。结果实验组HPV感染阳性者8例,感染率为15%,1例为HPV高、低危型混合感染,7例为HPV高危型单一感染;对照组HPV感染阳性者2例,感染率为1%,均为HPV高、低危型混合感染,两组HPV感染率比较差异有统计学意义(χ~2=37.5,P<0.05)。HPV阳性的OSCC病人中,Ⅰ、Ⅱ期者4例,Ⅲ、Ⅳ期者4例;HPV阴性的OSCC病人中,Ⅰ、Ⅱ期者25例,Ⅲ、Ⅳ期者20例,两组比较差异无统计学意义(P>0.05)。HPV阳性的OSCC病人中,有颈部淋巴结转移者3例,无转移者5例;HPV阴性的OSCC病人中,有颈部淋巴结转移者20例,无转移者25例,两组比较差异无统计学意义(P>0.05)。结论OSCC病人的HPV感染率较正常人群及口腔科其他疾病人群显著增高;HPV感染与OSCC的临床分期、淋巴结转移无相关性。

Objective To investigate the infection of human papillomavirus（HPV）in oral squamous cell carcinoma（SCC）. Methods Fifty-three patients with oral SCC treated in our department-between July 2014 and July 2016-were served as the experimental group.The cancer tissue,paracancerous tissue and normal oral mucosa were collected from the patients.Normal oral mucosa was taken from 50 patients with non-oral SCC treated in our department and 50 healthy volunteers-they were served as control group.The HPV infection of samples in the two groups was detected applying HPV DNA genotyping with PCRReverse Dot Blot hybridization technique. Results In the experimental group,HPV positive was found in eight（15%）cases,of which,one of mixed infection with high risk and low risk,and seven of HPV high risk single infection.In the control group,two cases（1.0%）were found of PPV positive,both of mixed infection with high and low risk,the difference in HPV infection rate between the two groups was significant（χ^2=37.5,P〈0.05）.In patients with oral SCC of positive HPV,four of stageⅠ-Ⅱ and four of stageⅢ-Ⅳwere found;of HPV negative,25 of stageⅠ-Ⅱ,20 of stageⅢ-Ⅳ,the difference was not significant between the two groups（P〉0.05）.In patients with oral SCC of HPV positive,cervical lymph node metastasis was found in three patients,and five without metastasis;those with oral SCC of negative HPV,20 were found with cervical lymph node metastasis,and25 without,the difference between the two groups was not significant（P〉0.05）. Conclusion The infection rate of HPV in patients with oral squamous cell carcinoma is higher than that in normal population and other oral-disease population.HPV infection is not correlated with clinical stage and lymphnode metastasis of oral squamous cell carcinoma.