正常人房角结构的激光共聚焦显微镜研究

Clinical research of anterior chamber angle of normol person by confocal microscope

目的 采用角膜激光共焦显微镜观察正常人房角超微组织学形态，分析房角各层结构在共聚焦显微镜下的表现，对房角结构研究拓展新的研究方法。方法 临床病例实验研究。对2014年11月至2015年6月在深圳市眼科医院普通门诊就诊的患者30例（30只眼），进行共聚焦显微镜检查，其中屈光不正15例占50%（15/30），结膜炎9例占30%（9/30），干眼症6例占20%（6/30），观察房角各层结构，包括Schwallbe线、小梁网，巩膜突及睫状体带结构，并对共聚焦显微镜获得的图像进行研究总结。结果 （1）通过共聚焦显微镜可观察到房角各层结构：30只眼（100%）均获得了房角Schwallbe线、小梁网的图像，14只眼（46.7%）获得了巩膜突结构的图像，3只眼（10%）获得了睫状体带结构的图像，1只眼（3.33%）获得了虹膜根部结构的图像。（2）各层结构在激光共聚焦显微镜下具有显著的特异性表现。结论 在三维空间实时、活体条件下对房角结构进行角膜激光共聚焦显微镜检查，可发现传统检查方法不能发现的超微变化。对房角结构研究扩展了崭新的研究途径。

Objective Toobserve the morphology of anterior chamber angle of normol person by confocal microscope, and the performance of each layer structure in the confocal microscope.Methods Thirty patients（30 eyes）with refractive errors or conjunctivitis or dry eyes by confocal microscopy were observed. The Schwallbe corner line, trabecular meshwork, scleralprotrusion and ciliary body structure were observed, and the confocal microscopy images were studied.Results Each layer of structure was observed by confocal microscope： there were 30 eyes obtained the images of Schwallbe corner line and the trabecular meshwork,accounted for 100%; 14 eyes obtained the images of sclera protrusion of the structure,accounted for 46.7%; 3 eyes obtained the images of ciliary body, accounted for 10%; and 1 eye received images of the iris root structure,accounted for 3.33%. The images of the layers of structure observed by laser confocal microscope had significant specificity.Conclusions In three-dimensional space in real time, the structure of the living conditions of anterior chamber angle structure by confocal microscopy can be found ultrastructural change compared with the traditional examination method that cannot be found.