二球悬铃木叶片挥发物与悬铃木方翅网蝽的相互作用研究

Volatile profiles of Platanus acerifolia leaves and their behavioral effects on Corythucha ciliata(Hemiptera:Tingidae)

[目的]为了理解悬铃木方翅网蝽Corythucha ciliata(Say)与二球悬铃木间的和昆虫与寄主互作机制,本实验测定了悬铃木方翅网蝽取食对其寄主植物二球悬铃木的挥发性化合物的影响,以及该虫对这些化合物的行为反应。[方法]运用固相微萃取结合气相色谱与质谱联用技术,测定了不同为害时间段中悬铃木叶片挥发物的变化。通过"Y"形嗅觉仪检测了该虫对不同处理的悬铃木叶片和其挥发物成分的行为反应。[结果]随着为害时间的增加,挥发性化合物的种类和数量总体呈上升趋势。悬铃木未处理叶片中的挥发物有4种成分,包括:顺-3-己烯醇,1,8-桉叶素,(E)-4,8-二甲基-1,3,7-壬三烯和反式-β-石竹烯。在为害后叶片中,共检测到9种挥发性化合物,除了上述4种成分外,新增加了α-侧柏烯,桧烯,月桂烯,顺-3-己烯醇乙酸酯和γ-松油烯。行为学研究发现,悬铃木叶片能显著地吸引悬铃木方翅网蝽,其中顺-3-己烯醇,1,8-桉叶素对该虫具有吸引作用,而反式-β-石竹烯具有趋避作用。[结论]悬铃木叶片被悬铃木方翅网蝽危害后会产生多种挥发物,其中一些挥发物能显著影响该虫的行为,可能在悬铃木方翅网蝽的寄主定位中起着重要的作用,另一些化合物可能参与悬铃木对该虫的间接防御反应。

[Objectives] The sycamore lace bug, Corythucha ciliata, is a newly found invasive insect in China. We examined the effects of C. ciliata feeding behavior on the volatile organic compounds （VOCs） of London plane tree （Platanus acerifolia） and the behavioral responses of C. ciliata to these VOCs. [Methods] We measured VOCs in the headspace of the P. acerifolia leaves damaged by C. ciliata using SPME/GC-MS. [Results] The number and quantity of VOCs generally increased with the duration of feeding by C. ciliata. Four chemicals, including cis-3-hexen-1-ol, 1, 8-cineole, （E）-4,8-dimethyl-1, 3, 7-nonatriene and trans-β-caryophyllene were detected in undamaged leaves. In addition, another five chemicals, including α-thujene, sabinene, myrcene, cis-3-hexenyl acetate and γ-terpinene were induced after feeding by C. ciliata. The behavioral responses of C. ciliata to P. acerifolia leaves and their volatile components were assayed in a Y-tube olfactometer. C. ciliata was significantly attracted by undamaged P. acerifolia leaves compared to a clean air control. Cis-3-hexen-1-ol and 1, 8-cineole significantly attracted C. ciliata, whereas trans-β-caryophyllene repelled this pest. [Conclusion] A blend of VOCs was detected after P. acerifolia leaves were damaged by C. ciliata and three compounds among these affected the behavior of this pest. These compounds may play an important role in host location by C. ciliata and in the indirect defensive response of P. acerifolia to C. ciliata.