坎地沙坦提高乏氧鼻咽癌CNE1细胞辐射敏感性

The study of the role of candesartan in radiosensitivity of nasopharyngeal carcinoma CNE1 cells

目的:探讨血管紧张素Ⅱ1型受体(ATIR)拮抗剂坎地沙坦对鼻咽癌CNE1细胞辐射抵抗性的影响。方法:噻唑蓝(MTT)比色法检测坎地沙坦对鼻咽癌CNE1细胞的生长抑制率;克隆形成实验检测坎地沙坦对常氧和乏氧条件下CNE1细胞放疗敏感性的影响;Western blot检测坎地沙坦对CNE1细胞中乏氧诱导因子1-α(HIF-1α)表达的影响。结果:在常氧和乏氧条件下,坎地沙坦均没有显著抑制CNE1细胞的生长,坎地沙坦在常氧条件没有显著增加CNE1细胞的辐射敏感性,然而坎地沙坦显著提高了乏氧CNE1细胞的辐射敏感性,坎地沙坦显著抑制了乏氧CNE1细胞中HIF-1α蛋白的表达。结论:坎地沙坦在体外并没有影响CNE1细胞的增殖能力,然而显著改善了乏氧CNE1细胞的辐射敏感性,其机制可能与其抑制了细胞内HIF-1α蛋白的表达有关。

Objective To explore the influence of candesartan （an angiotensin II receptorl antagonist, ATlR） in radioresistance of human nasopharyngeal carcinoma CNE1 cells. Methods Cell growth of CNE1 with or without candesartan treatment was measured in vitro by MTT method; radiosensitivity of CNE1 with or without candesartan treatment was tested under normoxic or hypoxie conditions by clone formation assay. The expression of hypoxia-induced factor 1α（HIF-1α）in CNE1 cells was analysed by western blotting. Results Candesartan did not significantly inhibit the growth of CNE1 cells in both normoxic and hypoxic conditions. Candesartan also did not influence the radiosensitivity of CNE1 cells in normoxic condition; however, it significantly increased the radiosensitivity of CNE1 ceils in hypoxic condition. The expression of hypoxia-induced factor 1 α （HIF-1α）in hypoxic CNE1 cells was significantly inhihited by candesartan treatment. Conclusion Candesartan does not significantly influence the proliferation of CNE1 cells in both normoxic and hypoxic conditions but significantly enhances the radiosensitivity of hypoxic CNE1 cells, in which the mechanism may be involved in its inhihiting HIF- 1α expression in hypoxic CNE1 cells.