KRT81 rs3660变异对肺癌发病风险的影响

Effect of KRT81 rs3660 Polymorphism on Risk of Lung Cancer

目的探讨KRT81基因3'UTR rs3660多态与肺癌遗传易感性之间的关系。方法使用Taq ManMGB荧光探针标记法对KRT81基因rs3660遗传变异进行基因分型。用Logistic回归计算rs3660各基因型影响非小细胞肺癌(NSCLC)和小细胞肺癌(SCLC)发病风险的OR值及95%CI。结果 KRT81基因rs3660G>C变异影响SCLC发病风险(P=0.048),但与NSCLC发病风险无关(P=0.614)。与rs3660GG基因型携带者相比,至少携带一个C等位基因者患SCLC风险显著降低(OR=0.70,95%CI:0.49~0.99)。吸烟分层分析显示,在不吸烟组中,至少携带一个C等位基因的非吸烟者具有较低的SCLC发病风险(OR=0.60,95%CI:0.36~0.99,P=0.049)。进一步以累计吸烟量来分析,未发现至少携带一个C等位基因的轻度吸烟者(或重度吸烟者)具有较低的SCLC发病风险(OR=0.61,95%CI:0.26~1.43,P=0.254)。结论 KRT81基因rs3660G>C变异影响SCLC发病风险,但与NSCLC发病风险无关。

Objective To explore the association of rs3660 polymorphism in the 3′UTR of KRT81 with the susceptibility for lung cancer. Methods The genotypes of rs3660 were determined by Taq Man-MGB realtime PCR. The OR and 95%CI were estimated by Logistic regression to evaluate the association of rs3660 polymorphism with the risk of non-small cell lung cancer（NSCLC） and small cell lung cancer（SCLC）. Results KRT81 rs3660G〉C variant affected the risk of SCLC（P=0.048）, not NSCLC（P=0.614）. When compared with the individuals with rs3660 GG genotype, a significant decreased risk of developing SCLC was shown in C allele carriers（OR=0.70, 95%CI： 0.49-0.99）. When stratified by smoking status, C allele carriers had significantly decreased risk among non-smokers（OR=0.60, 95%CI： 0.36-0.99, P=0.049）. We also found that C allele carriers had no significantly decreased risk among light smokers（or heavy smokers）（OR=0.61, 95%CI： 0.26-1.43, P=0.254）. Conclusion KRT81 rs3660G〉C polymorphism contributes to the risk of SCLC, not NSCLC.