甘肃不同产地黄芩的根和茎叶清热燥湿作用比较

Comparison the effect of clearing-heat and drying-damp on root and stem leaves of Scutellaria baicalensis from different areas in Gansu province

目的研究甘肃不同产地黄芩根、茎叶的清热燥湿作用异同。方法按照体重将SD大鼠随机分为空白组、模型组、陇西产黄芩Ⅰ组(根)、陇西产黄芩Ⅱ组(茎叶)、渭源产黄芩Ⅰ组(根)、渭源产黄芩Ⅱ组(茎叶)、漳县产黄芩Ⅰ组(根)、漳县产黄芩Ⅱ组(茎叶)、岷县产黄芩Ⅰ组(根)、岷县产黄芩Ⅱ组(茎叶),共10组。除空白组以外,其余各组大鼠给予肥甘饲料喂养和56度红星二锅头灌胃(5 mL·kg^(-1)),每日1次,并置于人工气候箱中,连续21 d;第22天,将浓度为1×10~9/mL大肠杆菌灌胃1次(10μL·g^(-1));4 h后,加强感染1次(5μL·g^(-1)),建立大鼠湿热证模型。空白组大鼠灌胃0.9%NaCl 10 mL·kg^(-1);各药物组大鼠,在模型制备的同时,分别给予黄芩根或茎叶水煎液10 g·kg^(-1)灌胃,均每天1次,连续7 d。观察各组大鼠证候学表现,以酶联免疫吸附法检测血清中白介素4(IL-4)、干扰素γ(IFN-γ)、胃动素(MTL)、胃泌素(GAS)的含量以及胃肠组织中MTL、GAS的含量。结果与模型组的IL-4、IFN-γ含量分别为(38.09±1.41),(1203.90±32.24)ng·L-1比较,各药物组大鼠血清中IL-4、IFN-γ的含量分别为(25.36±2.56),(703.53±29.36);(31.54.±3.81),(810.90±24.07);(26.88±2.91),(679.02±30.72);(30.81±3.19),(802.44±20.69);(26.61±3.94),(702.90±14.86);(32.45±5.12),(815.36±34.78);(27.06±4.01),(685.66±42.05);(31.76±3.98),(808.95±38.22)ng·L^(-1),差异均有统计学意义(均P<0.05)。与模型组GAS、MTL的含量分别为(28.61±0.12),(404.16±18.48)ng·L^(-1)比较,各药物组大鼠血清中GAS、MTL的含量分别为(34.14±1.47),(475.82±7.80);(35.12±3.40),(436.25±18.24);(34.77±2.65),(475.04±28.79);(33.28±3.58),(447.81±8.26);(35.26±4.89),(473.09±28.64);(36.02±5.12),(440.52±23.55);(35.20±4.55),(478.58±31.24);(34.88±5.07),(438.08±25.43)ng·L^(-1),差异均有统计学意义(均P<0.05)。结论甘肃不同产地黄芩根、茎叶水煎液均有一定的清热燥湿作用。

Objective To study the difference effects on clearing- heat and drying - damp of root and stem leaves of Scutellaria baicalensis from different areas in Gansu province. Methods One hundred SD rats were randomly divided into ten groups, including blank group, model group, Scuteliaria baicalensis from Longxi I and II groups, Weiyuan I and II groups, Zhangxian I and II groups,Minxian I and II groups, I group means root and II group means stem leaves, each group had ten rats. The blank group rats were given ordinary feed and saline（ 10 mL · kg^-1） in the natural environment. Rats in other groups were given fat feed and white wine [ Red Star Zhenpin Erguotou （56°） ,5 mL ·kg^-1] once daily, and placed into manual climatic box for 21 days. On the 22th day, the coli （ 1 × 10^9/mL） were given once （ 10μL· g^-1）, and enhancement of infection after 4 hours （5 μL· g^-1）. The animal models were given the roots and steams leaves of Scutellaria baicalensis decoction （ 10 g · kg^-1 ） by once daily with 7 days. The rats were placed into natural environment and given ordinary feed. The syndrome performance of rats in each group were observed. The contents of serum and stomach -intestine interleukin -4 （IL-4） ,interferon-γ（IFN -γ）, motilin（MTL） ,gastrin（GAS）in rats were detected by ELISA. Results With the treatment of the roots and steams leaves of Scutellaria baicalensis decoction, the serum contents of IL - 4 and IFN - γ were （25.36 ± 2.56）, （703.53 ± 29.36）; （31.54 ± 3.81）, （810.90 ± 24.07）; （26.88 ± 2.91）, （679. 02 ± 30. 72 ） ; （30. 81 ± 3. 19 ）, （ 802.44 ± 20. 69 ） ; （ 26. 61 ± 3. 94 ）, （ 702. 90 ± 14. 86 ） ; （ 32. 45 ± 5. 12 ）, （815.36 ± 34.78） ; （27.06 ± 4. 01 ）, （685.66 ± 42. 05 ） ; （31.76 ± 3.98 ）, （808.95 ± 38.22）ng · L^-1 respectively in each drugs group, which were obviously lower than that of model group with （ 38.09 ± 1.41 ） , （ 1203.90 ± 32.24 ） ng · L^-1 on contents of IL - 4 and IFN - γ. The contents of GAS and MTL in serum were （ 34. 14 ± 1.47 ） , （475.82±7.80）;（35.12 ±3.40）, （436.25±18.24）;（34.77 ±2.65）,（475.04 ±28.79）;（33.28 ±3.58）, （447.81 ±8.26） ; （35.26 ± 4. 89）, （473.09 ± 28.64） ; （36.02 ± 5.12）, （440. 52 ± 23.55） ; （35.20 ± 4. 55）, （478.58 ± 31.24） ; （34. 88 ± 5.07）, （438.08 ± 25.43） ng · L^-1 respectively in each drugs group, which were obviously higher than that of model group with （28.61 ±0. 12）, （404. 16 ± 18.48）ng· L^-1 on contents of GAS and MTL, all about with statistical difference （all P 〈0. 05）. Conclusion There was a significant effect on clearingheat and dryingdamp of root and stem leaves of Scutellaria baicalensis from different areas in Gansu province.