中国对虾虾壳制备几丁质工艺研究

Optimum technology of chitin preparation from penaeus chinensis

本实验以中国对虾虾壳为原料制备几丁质,利用柠檬酸脱钙、蛋白酶脱除蛋白质,通过对蛋白酶活力测定,本实验选用Alcalase碱性蛋白酶进行实验。通过单因素实验和Box-behnken响应面设计优化几丁质的最佳制备条件,以灰分含量为脱钙指标,得到柠檬酸脱钙最佳工艺条件为:固液比1∶12,反应时间2.5 h,柠檬酸浓度为8%,灰分含量为0.42%。以蛋白质含量为指标,测得碱性蛋白酶脱蛋白的最佳工艺条件为:加酶量2200 U/g,反应时间5 h,反应温度50℃,脱除的蛋白质浓度可达到31.42%。本实验条件温和、反应迅速、无环境污染,符合我国绿色无公害理念,为工业化生产几丁质提供基础。

In this study,chitin was extracted from Penaeus ehinensis,and the protein was removed by protease and decaMfied by citric acid.The protease activity was measured by Alcalase Alkaline protease. The optimal conditions of chitin preparation were determined by single factor experiment and Box- behnken response surface design. The optimal conditions of decalcification were obtained as follows： solid-liquid ratio 1 ： 12, reaction Time 2.5 h, citric acid concentration of 8% , ash content of 0.42%.The optimum conditions were as follows：adding 2200 U/g enzyme, reaction time 5 h, reaction temperature 50 ℃ ,and the protein concentration could reach 31.42% by taking the protein content as the index. The experimental conditions are mild, rapid response, no environmental pollution, in line with the concept of pollution-free green, and to provide the basis for industrial production of chitin.