苦丁茶粗多酚对DSS诱导C57BL/6J小鼠溃疡性结肠炎的预防作用

Preventive effects of Kuding tea crude polyphenols in DSS-induced C57BL/6J mice ulcerative colitis

目的:通过DSS(葡聚糖硫酸钠)诱导溃疡性结肠炎(UC)动物模型对苦丁茶粗多酚(KTCP)的UC预防效果进行了研究。方法:使用试剂盒检测小鼠的血清指标,同时使用RT-PCR技术检测小鼠结肠组织的mRNA表达。结果:动物实验结果显示KTCP能控制UC造成的小鼠体重下降和疾病活动指数(Disease activity index,DAI)增高;同时,KTCP还可以使UC造成结肠长度缩短和结肠重量/结肠长度比值降低得到有效的控制。解剖小鼠后对小鼠的血清和组织进行实验,观察到KTCP可以提高血清中SOD(超氧化物歧化酶)含量、降低细胞因子IL-6(白介素-6)、IL-12(白介素-12)和TNF-α(肿瘤坏死因子-α)的水平;KTCP还可以降低结肠组织中MPO(髓过氧化物酶)、MDA(丙二醛)的含量、提高GSH(谷胱甘肽)的含量。RT-PCR实验结果显示,相对于对照组KTCP可以下调结肠组织中TNF-α、IL-1β、IL-6、IL-8和CXCR2的mRNA表达。同时,高浓度的KTCP使小鼠的实验指标更接近于正常组小鼠,显示出更好的效果。结论:KTCP具有较好的溃疡性结肠炎的预防作用。

Objective：The preventive effects of Kuding tea crude polyphenol （KTCP）on ulcerative colitis were determined through the DSS（ Dextran sulfate sodium salt）-induced ulcerative colitis（UC） animal mode/ in this study.Method：Experiment kits were used for the mice serum determination, meanwhile, the mRNA of mice colon tissue were determined by RT- PCR assay.Results：The animal experiment results showed that KTCP could control the UC caused mice body weight reduction and DAI（ disease activity index）raise.Meantime, KTCP also could control the UC caused colon length shortening and colon ratio of weight/colon length reduction.After dissection ,the serum and tissue of mice were experimentalized, it was observed that KTCP could increase the content of SOD （ superoxide dismutase ） in serum, decrease the cytokine levels of IL- 6 （ interleukin- 6 ） , IL- 12 （ interleukin- 12 ） and TNF- α （ tumor necrosis factor alpha）. And it was also observed that KTCP could reduce the MPO （myeloperoxidase）, MDA （ malondialdehyde ） contents and raise the GSH （ glutathione ） content in colon tissues. RT- PCR experiment results showed that KTCP could decrease the TNF- α, IL- 1/3, IL- 6, IL- 8 and CXCR2 mRNA in colon tissues compared to the control group mice. Meanwhile high concentration of KTCP could make the experiment index close to the normal group mice,and showed the better activities.Conclusion：As can be seen,KTCP had a good preventive effects of UC.