糖痹康对高糖环境下大鼠雪旺细胞NF-κB表达的影响

Effect of Tangbikang on expression of Schwann cells of rats with NF-κB in environment of strong concentration glucose

目的观察中药糖痹康对高糖环境下大鼠雪旺细胞NF-κB表达的影响。方法采用原代培养的方法建立高糖环境下大鼠雪旺细胞的模型,用高、中、低三种剂量糖痹康含药血清进行干预,以弥可保作为对照,用Western blot检测不同药物干预后的大鼠雪旺细胞NF-κB p65蛋白表达,实时荧光定量PCR技术检测雪旺细胞中NF-κB mRNA的表达。结果与正常对照组相比,模型组NF-κB p65蛋白相对表达量明显减弱,与模型组相比,各给药组NF-κB蛋白的表达均有所升高,并有统计学意义(P<0.05);与正常组相比,模型组NF-κB mRNA相对表达量明显减弱,与模型组相比,各给药组NF-κB mRNA的表达均有所升高,并有统计学意义(P<0.05)。结论糖尿病周围神经病变时NF-κB表达异常增加,中药糖痹康能够减少NF-κB活化,降低NF-κB转录水平,这可能是糖痹康对糖尿病周围神经病变(DPN)神经保护的有效作用靶点之一。

Objective To investigate the effect of traditional Chinese medicine tangbikang （TBK） on expression of Schwann cells of rats with NF - κB in the environment of high concentration glucose. Methods Establish model of rat Schwann cells in the environment of high concentration glucose by using primary culture with three kinds of high, medium and low doses of TBK drug - containing serum intervention. The control group is given the mecobalamin. Applying west- eru blot to detect the expression of rat Schwann cells NF - κBp65 protein were intervened by different drugs intervention. And using real - time fluorescent quantification PCR technology to test the Schwann cells NF - κB mRNA＇ expression. Results The relative quantitative expression of NF - κB p65 protein and NF - κB mRNA weakened apparently com- pared with normal controls;The quantitative expression of NF - κB 1365 protein and NF - κB mRNA of drugs - given groups were increased compared with model groups, with statistical significance. Conclusion The expression of NF - κB increase abnormally when it＇ s in DPN state. Traditional Chinese medicine TBK is able to reduce NF - κB activation,lower the transcriptional level of NF - κB. It may be one of effective targets that TBK apply on DPN nerve production.