甘肃省敦煌地区普通人群卡波肉瘤相关疱疹病毒感染的血清学分析

Seroprevalence of Kaposi's sarcoma-associated herpesvirus in Dunhuang,Gansu province

目的研究卡波肉瘤相关疱疹病毒(KSHV)在甘肃敦煌地区普通人群中的血清阳性率,并分析KSHV感染的危险因素。方法随机收集1 000例敦煌地区普通人群血清,以KSHV 3个基因片段orf65、orf73、orf k8.1编码蛋白为抗原,间接ELISA检测样本中KSHV抗体,SPSS软件分析危险因素。结果 1 000份血清中,KSHV抗体总阳性率为19%,男性阳性率(22.9%)高于女性(16.6%)(P=0.016);KSHV感染与梅毒螺旋体(P=0.004)和丙肝病毒(P=0.027)感染间有相关性,而与年龄、乙肝五项无关。多因素Logistic回归分析显示梅毒螺旋体感染的OR(95%CI)为10.142(1.920~53.577)。结论甘肃敦煌地区普通人群中KSHV的感染率较高,且与性别有关;梅毒螺旋体感染是该地区KSHV感染最重要的独立危险因素。

Objective To estimate the seroprevalence and risk factors for Kaposi＇ s sarcoma-associated herpesvirus （KSHV） infection in the general population in Dunhuang, Gansu province. Methods We examined 1 000 sera specimens using an EL1SA-based assay that measured antibodies to one KSHV latent antigen （ORF73） and two KSHV lytic proteins （ ORF65 and ORF K8. 1 ）. Results Of the 1000 subjects, the seroprevalence of KSHV in the general population was 19% and slightly higher in the male than that in the female （22.9% vs 16.6%, P = 0. 016）. Statistical analysis showed that seroprevalence of KSHV had significant associations with syphilis （P=0. 004） and hepatitis C virus infection （P = 0. 027 ）, but it had no differences with the age, hepatitis B virus infec- tion. Conclusion These results indicate that the infection rates of KSHV in Dunhuang, Gansu province are higher than that in other areas in China. The infection of KSHV is significantly associated with the gender. Syphilis infec- tion is the most important independent risk factor for KSHV infection in this area.