基于线粒体COⅠ基因的海陵岛鲷科鱼类系统发育分析

Phylogenetic Analysis of Family Sparidae Derived from Hailing Island Based on Mitochondrial COⅠ Gene Sequences

对采自海陵岛的6种鲷科鱼类(Sparidae)86个个体的线粒体COⅠ基因进行测序,结合Gen Bank上的17条同源序列,共7属11种103个个体进行系统进化分析。海陵岛11种鲷科鱼类COⅠ基因序列的T、C、A、G的平均含量分别为31.9%、27.6%、23.4%和17.1%,有保守位点359个,变异位点165个,简约信息位点162个,序列中转换大于颠换。Kimura 2-parameter种间遗传距离显示,真赤鲷和二长棘犁齿鲷的遗传距离小于前者与蓝点赤鲷的遗传距离。应用邻接法(NJ法)和最大似然法(ML法)对103条序列分别构建了鲷科鱼类的分子系统进化树。结果显示,鲷科鱼类分为两大类群,一类包括犁齿鲷属、赤鲷属、牙鲷属和四长棘鲷属,另一类包括平鲷属、棘鲷属和拟牛眼鲷属。根据种间遗传距离与系统发育树的结构特征推测,真赤鲷与二长棘犁齿鲷可能属于同一属,这与传统的形态分类学观点不完全相符。

The mitochondrial CO I gene of 86 specimens of 6 species of Sparidae collected from Hailing Island were sequenced. Combined with 17 homologous sequences on GenBank, a total of 103 individuals of 7 genus and 11 species were analyzed phylogenetically in this study. The results indicated that the average contents of T, C, A and G were 31.9%, 27.6%, 23.4% and 17.1%, respectively in the 11 Sparidae species from Hailing Island, which contained 359 conserved sites, 165 variable sites and 162 parsimony informative polymorphic sites, and the con- version in the sequence was greater than the transversion. The interspecific genetic distance of Kimura 2-parameter showed that the genetic distance between Pagrus major and Evynnis eardinalis was shorter than that between the former one and Pagrus caeruleostictus. The neighbour-joining （NJ） and maximum-likelihood （ML） methods were used to construct molecular phylogenetic tree of 103 sequences of Sparidae, which revealed that Family Sparidae could be divided into two clades： one clade included Dentex, Argyrops, Evynnis, and Pogrus, and the other clade included Rhabdosargus, Aeanthopagrus, and Boopsoidea. According to the genetic distance between species and the structural characteristics ofphylogenetic tree, we predicted that Pagrus major and Evynnis cardina- lis might belong to the same genera, which was not consistent with the traditional morphological taxonomy.